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通过金纳米颗粒-单壁碳纳米管生物共轭纳米杂化物对多重耐药性肠炎沙门氏菌DT104进行靶向高灵敏度检测/根除。

Targeted highly sensitive detection/eradication of multi-drug resistant Salmonella DT104 through gold nanoparticle-SWCNT bioconjugated nanohybrids.

作者信息

Lin Yunfeng, Hamme Ii Ashton T

机构信息

Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS 39217, USA.

出版信息

Analyst. 2014 Aug 7;139(15):3702-5. doi: 10.1039/c4an00744a.

Abstract

Monoclonal antibody-conjugated sphere-shaped gold nanoparticles were combined with single-walled carbon nanotubes (SWCNTs) to create a nanohybrid system to selectively detect and eradicate multiple drug resistant Salmonella (MDRS) typhimurium DT104 bacteria. The Raman signal intensity from Rhodamine 6G (Rh6G) modified monoclonal AC04 antibody SWCNTs-gold nanoparticle (SWCNT-GNPs) hybrid provided a SERS enhancement by several orders of magnitude to detect the MDRS bacteria over the GNP system. A targeted photothermolysis experiment using 670 nm light at 2 W cm(-2) for 15 min, resulted in selective and irreparable damage to more than 99% Salmonella DT104 at the concentration of 10(5) CFU mL(-1). In comparison to solely SWCNTs or GNPs, our SWCNT-GNPs nanohybrids have also shown a better photothermal efficiency.

摘要

将单克隆抗体偶联的球形金纳米颗粒与单壁碳纳米管(SWCNTs)相结合,构建了一种纳米杂化系统,用于选择性检测和根除多重耐药鼠伤寒沙门氏菌(MDRS)DT104细菌。罗丹明6G(Rh6G)修饰的单克隆AC04抗体SWCNT-金纳米颗粒(SWCNT-GNPs)杂化物的拉曼信号强度比GNP系统提供了几个数量级的表面增强拉曼散射(SERS)增强,以检测MDRS细菌。使用670 nm光、2 W cm(-2)照射15分钟的靶向光热解实验,在10(5) CFU mL(-1)的浓度下,对超过99%的沙门氏菌DT104造成了选择性且不可修复的损伤。与单独的SWCNTs或GNPs相比,我们的SWCNT-GNPs纳米杂化物也表现出了更好的光热效率。

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