Martín Rocío, Delgado Susana, Maldonado Antonio, Jiménez Esther, Olivares Mónica, Fernández Leónides, Sobrino Odón J, Rodríguez Juan M
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
J Dairy Res. 2009 Nov;76(4):418-25. doi: 10.1017/S0022029909990124. Epub 2009 Jul 29.
Sow milk protects the piglet against infectious diseases through a variety of mechanisms. In this study, the presence of potentially probiotic lactic acid bacteria in this biological fluid was investigated. Milk samples were obtained from 8 sows and a total of 19 rod-shaped isolates were selected for identification and assessment of their probiotic potential. RAPD profiling revealed the existence of 8 different genetic profiles among them. One representative of each profile was selected for further characterization and they were identified as Lactobacillus reuteri, Lb. salivarius, Lb. plantarum, Lb. paraplantarum, Lb. brevis and Weissella paramesenteroides. Then, their probiotic potential was evaluated through different assays, including survival in conditions simulating those existing in the gastrointestinal tract, production of antimicrobial compounds, adherence to intestinal mucin, production of biogenic amines, degradation of mucin, and pattern of antibiotic sensitivity. Three strains, Lb. reuteri CR20 (a reuterin-producing strain), Lb. salivarius CELA2 (a bacteriocin-producing strain) and Lb. paraplantarum CLB7 displayed the highest probiotic potential.
母猪乳汁通过多种机制保护仔猪免受传染病侵害。在本研究中，对这种生物液体中潜在的益生菌乳酸菌的存在情况进行了调查。从8头母猪采集了乳汁样本，共选择了19株杆状分离株进行鉴定并评估其益生菌潜力。随机扩增多态性DNA分析显示其中存在8种不同的遗传图谱。每种图谱选择一个代表进行进一步表征，它们被鉴定为罗伊氏乳杆菌、唾液乳杆菌、植物乳杆菌、副植物乳杆菌、短乳杆菌和类肠膜魏斯氏菌。然后，通过不同的试验评估它们的益生菌潜力，包括在模拟胃肠道现有条件下的存活能力、抗菌化合物的产生、对肠粘蛋白的粘附、生物胺的产生、粘蛋白的降解以及抗生素敏感性模式。三株菌，即罗伊氏乳杆菌CR20（产罗伊氏菌素菌株）、唾液乳杆菌CELA2（产细菌素菌株）和副植物乳杆菌CLB7表现出最高益生菌潜力。
