Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Vet J. 2010 Aug;185(2):193-8. doi: 10.1016/j.tvjl.2009.04.014. Epub 2009 May 17.
Canine milk protects puppies against infectious diseases through a variety of mechanisms. In this study, the presence of potentially probiotic lactic acid bacteria grown on MRS-Cys agar plates from milk of nine bitches was investigated. The Gram positive catalase negative bacilli identified in this study belonged to four Lactobacillus species (Lactobacillus reuteri, Lactobacillus fermentum, Lactobacillus murinus, Lactobacillus animalis), as well as one isolate that was identified as Weissella viridescens. Random amplified polymorphic DNA profiling revealed 28 different genetic profiles among the lactobacilli isolates. Their probiotic potential was evaluated through different assays, including survival in conditions that resemble those existing in the gastrointestinal tract, production of antimicrobial compounds, adherence to intestinal mucin, degradation of mucin and pattern of antibiotic sensitivity. Some strains showed potential for future applications as canine probiotics.
犬奶通过多种机制保护幼犬免受传染病的侵害。在这项研究中，从 9 只母犬的奶中在 MRS-Cys 琼脂平板上生长的潜在益生菌乳酸菌的存在情况进行了研究。在这项研究中鉴定出的革兰氏阳性过氧化氢酶阴性杆菌属于四种乳酸菌（鼠李糖乳杆菌、发酵乳杆菌、乳酸乳球菌、乳双歧杆菌），以及一个被鉴定为韦荣球菌的分离株。随机扩增多态性 DNA 分析显示，乳酸菌分离株中有 28 种不同的遗传特征。通过不同的试验评估了它们的益生菌潜力，包括在类似于胃肠道中存在的条件下的生存能力、产生抗菌化合物、对肠道粘蛋白的黏附性、粘蛋白的降解和抗生素敏感性模式。一些菌株显示出作为犬用益生菌的应用潜力。
