Department of Biochemistry, Shivaji University, Kolhapur 416004, India.
J Hazard Mater. 2009 Dec 15;172(1):298-309. doi: 10.1016/j.jhazmat.2009.07.008. Epub 2009 Jul 8.
An isolated bacterial strain is placed in the branch of the Bacillus genus on the basis of 16S rRNA sequence and biochemical characteristics. It decolorized an individual and mixture of dyes, including reactive, disperse and direct. Bacillus sp. ADR showed 88% decolorization of sulfonated azo dye C.I. Reactive Orange 16 (100 mg L(-1)) with 2.62 mg of dye decolorized g(-1) dry cells h(-1) as specific decolorization rate along with 50% reduction in COD under static condition. The optimum pH and temperature for the decolorization was 7-8 and 30-40 degrees C, respectively. It was found to tolerate the sulfonated azo dye concentration up to 1.0 g L(-1). Significant induction in the activity of an extracellular phenol oxidase and NADH-DCIP reductase enzymes during decolorization of C.I. Reactive Orange 16 suggest their involvement in the decolorization. The metal salt (CaCl2), stabilizers (3,4-dimethoxy benzyl alcohol and o-tolidine) and electron donors (sodium acetate, sodium formate, sodium succinate, sodium citrate and sodium pyruvate) enhanced the C.I. Reactive Orange 16 decolorization rate of Bacillus sp. ADR. The 6-nitroso naphthol and dihydroperoxy benzene were final products obtained after decolorization of C.I. Reactive Orange 16 as characterized using FTIR and GC-MS.
基于 16S rRNA 序列和生化特性，将一株分离的细菌菌株归属于芽孢杆菌属的分支。该菌株能够对包括活性染料、分散染料和直接染料在内的单一和混合染料进行脱色。芽孢杆菌 ADR 对磺化偶氮染料 C.I. 活性橙 16（100mg/L）的脱色率为 88%，以特定脱色率计，每克干细胞每小时可脱色 2.62mg 染料，同时在静态条件下，COD 降低 50%。该菌株对染料的最佳 pH 值和温度分别为 7-8 和 30-40°C。它能够耐受高达 1.0g/L 的磺化偶氮染料浓度。在 C.I. 活性橙 16 脱色过程中，胞外酚氧化酶和 NADH-DCIP 还原酶的活性显著增加，表明它们参与了脱色过程。金属盐（CaCl2）、稳定剂（3,4-二甲氧基苄醇和邻甲苯胺）和电子供体（乙酸钠、甲酸钠、丁酸钠、柠檬酸钠和丙酮酸钠）均能提高芽孢杆菌 ADR 对 C.I. 活性橙 16 的脱色速率。用 FTIR 和 GC-MS 对 C.I. 活性橙 16 脱色后的产物进行了表征，结果表明，6-亚硝基萘酚和二氢过氧苯是最终产物。
