Xue Zhaohui, Liu Zhiwei, Wu Moucheng, Zhuang Shiwen, Yu Wancong
School of Agriculture and Bioengineering, Tianjin University, Tianjin 300072, PR China.
Exp Toxicol Pathol. 2010 Sep;62(5):519-23. doi: 10.1016/j.etp.2009.06.013. Epub 2009 Jul 28.
Rapeseed peptide (RSP), obtained by hydrolyzing rapeseed protein, has anticancer activity. In this study, the effects of RSP on proliferation rate, morphological changes, DNA damage, cell cycle distribution and apoptosis in human cervical carcinoma (Hela) cells were investigated. RSP treatment at a concentration of 640 mg/L for 4 days inhibited Hela cell proliferation significantly, as determined by the MTT assay. We observed a dose-dependent increase in cytotoxicity induced by RSP at 20-640 mg/L. After 4 days of 320 mg/L RSP treatment, typical apoptotic changes were observed by transmission electron microscopy (TEM). Using the comet assay, we found dramatic comet tails, indicating DNA damage by RSP (20-640 mg/L). Moreover, RSP treatment caused inhibition of Hela cell growth, with cycle arrest in the S phase and apoptosis induction. Taken together, the results suggested that rapeseed peptide could be a potential antitumor compound with an apoptotic mode of action.
通过水解菜籽蛋白获得的菜籽肽（RSP）具有抗癌活性。在本研究中，研究了RSP对人宫颈癌（Hela）细胞增殖率、形态变化、DNA损伤、细胞周期分布和凋亡的影响。通过MTT法测定，640 mg/L浓度的RSP处理4天可显著抑制Hela细胞增殖。我们观察到20 - 640 mg/L的RSP诱导的细胞毒性呈剂量依赖性增加。320 mg/L RSP处理4天后，通过透射电子显微镜（TEM）观察到典型的凋亡变化。使用彗星试验，我们发现了明显的彗星尾，表明RSP（20 - 640 mg/L）造成了DNA损伤。此外，RSP处理导致Hela细胞生长受到抑制，细胞周期停滞在S期并诱导凋亡。综上所述，结果表明菜籽肽可能是一种具有凋亡作用模式的潜在抗肿瘤化合物。
