National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China.
Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China.
Int J Mol Sci. 2019 Oct 31;20(21):5425. doi: 10.3390/ijms20215425.
In our previous research, ten antioxidant pentapeptides including FYKWP, FTGMD, GFEPY, YLPYA, FPPYERRQ, GFYAA, FSGLR, FPYLRH, VPDDD, and GIEWA were identified from the hydrolysate of miiuy croaker ( ) swim bladder. In this work, their protective function on HO-induced oxidative damage to human umbilical vein endothelial cells (HUVECs) was studied. Results indicated that there was no significant difference in the HUVEC viability between the normal group and the treated groups with the 10 pentapeptides at the concentration of 100 μM for 24 h ( < 0.05). Furthermore, FPYLRH of 100 μg/mL extremely significantly ( < 0.001) increased the viability (80.58% ± 5.01%) of HUVECs with HO-induced oxidative damage compared with that of the model group. The protective mechanism indicated that FPYLRH could extremely significantly ( < 0.001) increase the levels of superoxide dismutase (SOD) (211.36 ± 8.29 U/mg prot) and GSH-Px (53.06 ± 2.34 U/mg prot) and decrease the contents of reactive oxygen species (ROS) (139.1 ± 11.8% of control), malondialdehyde (MDA) (13.66 ± 0.71 nM/mg), and nitric oxide (NO) (4.36 ± 0.32 µM/L) at the concentration of 100 μM in HUVECs with HO-induced oxidative damage compared with those of the model group. In addition, FPYLRH dose-dependently protected DNA in oxidative damage HUVECs model. These results suggested that FPYLRH could significantly attenuate the HO-induced stress injury in HUVECs and might be used as a potential natural antioxidant in the functional food industries.
在我们之前的研究中，从米鱼鳔水解物中鉴定出了十种抗氧化五肽，包括 FYKWP、FTGMD、GFEPY、YLPYA、FPPYERRQ、GFYAA、FSGLR、FPYLRH、VPDDD 和 GIEWA。在这项工作中，研究了它们对 HO 诱导的人脐静脉内皮细胞（HUVEC）氧化损伤的保护作用。结果表明，在 100μM 浓度下，10 种五肽处理组与正常组之间的 HUVEC 活力无显著差异（<0.05）。此外，与模型组相比，浓度为 100μg/mL 的 FPYLRH 可极显著（<0.001）提高 HO 诱导氧化损伤的 HUVEC 活力（80.58%±5.01%）。保护机制表明，FPYLRH 可极显著（<0.001）提高超氧化物歧化酶（SOD）（211.36±8.29U/mgprot）和谷胱甘肽过氧化物酶（GSH-Px）（53.06±2.34U/mgprot）的水平，降低活性氧（ROS）（139.1±11.8%的对照）、丙二醛（MDA）（13.66±0.71nM/mg）和一氧化氮（NO）（4.36±0.32µM/L）的含量，与模型组相比，HO 诱导氧化损伤的 HUVEC 中。此外，FPYLRH 以剂量依赖的方式保护氧化损伤 HUVEC 模型中的 DNA。这些结果表明，FPYLRH 可显著减轻 HO 诱导的 HUVEC 应激损伤，可能作为功能性食品工业中潜在的天然抗氧化剂。
