Department of Materials, Imperial College London, London SW7 2BP, UK.
J R Soc Interface. 2010 Mar 6;7(44):453-65. doi: 10.1098/rsif.2009.0255. Epub 2009 Jul 29.
This work investigated the effect of adding nanoparticulate (29 nm) bioactive glass particles on the bioactivity, degradation and in vitro cytocompatibility of poly(3-hydroxybutyrate) (P(3HB)) composites/nano-sized bioactive glass (n-BG). Two different concentrations (10 and 20 wt %) of nanoscale bioactive glass particles of 45S5 Bioglass composition were used to prepare composite films. Several techniques (Raman spectroscopy, scanning electron microscopy, atomic force microscopy, energy dispersive X-ray) were used to monitor their surface and bioreactivity over a 45-day period of immersion in simulated body fluid (SBF). All results suggested the P(3HB)/n-BG composites to be highly bioactive, confirmed by the formation of hydroxyapatite on material surfaces upon immersion in SBF. The weight loss and water uptake were found to increase on increasing bioactive glass content. Cytocompatibility study (cell proliferation, cell attachment, alkaline phosphatase activity and osteocalcin production) using human MG-63 osteoblast-like cells in osteogenic and non-osteogenic medium showed that the composite substrates are suitable for cell attachment, proliferation and differentiation.
这项工作研究了添加纳米（29nm）生物活性玻璃颗粒对聚（3-羟基丁酸酯）（P（3HB））复合材料/纳米生物活性玻璃（n-BG）的生物活性、降解和体外细胞相容性的影响。使用两种不同浓度（10 和 20wt%）的 45S5 Bioglass 组成的纳米生物活性玻璃颗粒来制备复合膜。使用多种技术（拉曼光谱、扫描电子显微镜、原子力显微镜、能量色散 X 射线）在模拟体液（SBF）中浸泡 45 天期间监测其表面和生物反应性。所有结果表明 P（3HB）/n-BG 复合材料具有很高的生物活性，这通过在 SBF 中浸泡在材料表面上形成羟基磷灰石得到证实。随着生物活性玻璃含量的增加，发现重量损失和吸水率增加。使用成骨和非成骨培养基中的人 MG-63 成骨样细胞进行细胞相容性研究（细胞增殖、细胞附着、碱性磷酸酶活性和骨钙素产生）表明复合基质适合细胞附着、增殖和分化。
