Tsai Shih-Meng, Lin Shu-Kai, Lee King-The, Hsiao Jen-Kuei, Huang Jung-Chih, Wu Szu-Hsien, Ma Hsu, Wu Szu-Hwa, Tsai Li-Yu
Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Ann Clin Biochem. 2009 Sep;46(Pt 5):394-400. doi: 10.1258/acb.2009.009029. Epub 2009 Jul 29.
Excess reactive oxygen species related to neoplasia of liver has been established. Essentially, the human body has developed different antioxidant systems for defence against these attacks. To evaluate the redox status in hepatocellular carcinoma (HCC) induced by hepatitis B virus (HBV), the most important aetiological factor in Taiwan, changes in O2(.) generation, lipid peroxidation as well as antioxidant status in the blood of HCC patients with HBV carriers for more than 20 years were measured.
Superoxide anion radical (O2(.-)) generation and the levels of malondialdehyde (MDA) served as an index of lipid peroxidation along with the analyses of activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRx); also, glutathione status, including reduced glutathione (GSH) and oxidized glutathione (GSSG), and the levels of vitamins A, C and E were determined.
In 54 patients, the levels of O2(.-), MDA and GSSG, and the activities of SOD and GRx of blood were significantly higher than those of 57 controls. Conversely, the levels of GSH and total GSH, and GSH/GSSG ratio, and vitamins A and C were significantly decreased. Additionally, there were no significant changes in the activity of GPx and the levels of vitamin E.
Our data suggest that the redox statuses in patients with HBV-associated HCC were elevated or decreased in certain parameters. However, the increased activities of antioxidant enzymes may be a compensatory up-regulation and the decrease antioxidant statuses were responses to the enhanced oxidative stress in those patients.
与肝癌形成相关的活性氧过量已得到证实。本质上，人体已发展出不同的抗氧化系统来抵御这些攻击。为评估由台湾最重要的病因——乙型肝炎病毒（HBV）诱导的肝细胞癌（HCC）中的氧化还原状态，我们检测了HBV携带者超过20年的HCC患者血液中O2(.)生成、脂质过氧化以及抗氧化状态的变化。
超氧阴离子自由基（O2(.-)）生成以及丙二醛（MDA）水平作为脂质过氧化指标，同时分析超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GRx）的活性；此外，还测定了谷胱甘肽状态，包括还原型谷胱甘肽（GSH）和氧化型谷胱甘肽（GSSG），以及维生素A、C和E的水平。
在54例患者中，血液中O2(.-)、MDA和GSSG水平以及SOD和GRx活性显著高于57例对照。相反，GSH和总GSH水平、GSH/GSSG比值以及维生素A和C水平显著降低。此外，GPx活性和维生素E水平无显著变化。
我们的数据表明，HBV相关HCC患者的某些参数氧化还原状态升高或降低。然而，抗氧化酶活性增加可能是一种代偿性上调，而抗氧化状态降低是这些患者对增强的氧化应激的反应。
