Vezzani A, Serafini R, Samanin R, Foster A C
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Brain Res. 1990 Aug 27;526(1):113-21. doi: 10.1016/0006-8993(90)90256-b.
Binding sites for excitatory amino acids have been determined by autoradiographical procedures in the rat hippocampus and striatum during hippocampal kindling. The binding sites measured were the N-methyl-D-aspartate (NMDA)-sensitive sites for L-[3H]glutamate and [3H]MK-801 sites (transmitter recognition site and ion channel of the NMDA receptor, respectively), [3H]alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) sites (quisqualate receptor), [3H]kainate sites (kainate receptor) and NMDA-insensitive sites for L-[3H]glutamate. In general, little change was apparent in the hippocampus or striatum for any of these binding sites when assessed 48 h after attaining stages 1/2, 3 or 5 of kindling. These results suggest that hippocampal kindling does not bring about a change in the excitatory amino acid receptor binding sites examined, and that the appearance of an NMDA receptor-mediated component to synaptic responses in the hippocampus produced by kindling, cannot be explained on this basis.
在海马体点燃过程中，通过放射自显影术已确定了大鼠海马体和纹状体中兴奋性氨基酸的结合位点。所测量的结合位点分别是L-[3H]谷氨酸的N-甲基-D-天冬氨酸（NMDA）敏感位点和[3H]MK-801位点（分别为NMDA受体的递质识别位点和离子通道）、[3H]α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）位点（quisqualate受体）、[3H]海人藻酸位点（海人藻酸受体）以及L-[3H]谷氨酸的NMDA不敏感位点。一般来说，在达到点燃的1/2期、3期或5期48小时后进行评估时，海马体或纹状体中这些结合位点中的任何一个都没有明显变化。这些结果表明，海马体点燃不会导致所检测的兴奋性氨基酸受体结合位点发生变化，并且点燃在海马体中产生的突触反应中NMDA受体介导成分的出现，无法基于此得到解释。
