苯甲酸对人外周血淋巴细胞的体外遗传毒性。
The in vitro genotoxicity of benzoic acid in human peripheral blood lymphocytes.
作者信息
Yılmaz Serkan, Ünal Fatma, Yüzbaşıoğlu Deniz
机构信息
Health Sciences Faculty, Department of Midwifery, Ankara University, 06100, Ankara, Turkey.
Arts and Science Faculty, Department of Biology, Gazi University, Teknikokullar, 06500, Ankara, Turkey.
出版信息
Cytotechnology. 2009 Jul;60(1-3):55. doi: 10.1007/s10616-009-9214-z. Epub 2009 Jul 30.
Abstract
The chromosomal aberration (CA), sister chromatid exchange (SCE) and micronucleus test (MN) were employed to investigate the in vitro effect of antimicrobial food additive benzoic acid on human chromosomes. Lymphocytes were incubated with various concentrations (50, 100, 200 and 500 μg/mL) of benzoic acid. The results of used assays showed that benzoic acid significantly increased the chromosomal aberration, sister chromatid exchange and micronucleus frequency (200 and 500 μg/mL) without changing the pH of the medium in a dose-dependent manner. Also this additive significantly decreased the mitotic index (MI) at the highest concentration for 24 h and 100, 200 and 500 μg/mL for 48 h. This decrease was dose-dependent as well. However, it did not effect the replication (RI) and nuclear division (NDI) indices.
摘要
采用染色体畸变（CA）、姐妹染色单体交换（SCE）和微核试验（MN）来研究抗菌食品添加剂苯甲酸对人类染色体的体外作用。将淋巴细胞与不同浓度（50、100、200和500μg/mL）的苯甲酸一起孵育。所使用试验的结果表明，苯甲酸显著增加了染色体畸变、姐妹染色单体交换和微核频率（200和500μg/mL），且呈剂量依赖性，同时培养基的pH值未发生变化。此外，该添加剂在最高浓度（24小时为500μg/mL，48小时为100、200和500μg/mL）时显著降低了有丝分裂指数（MI）。这种降低也是剂量依赖性的。然而，它对复制指数（RI）和核分裂指数（NDI）没有影响。
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