用γ射线辐照的罗素蝰蛇毒类毒素对兔子进行主动免疫。

Active immunization of rabbit with gamma irradiated Russell's viper venom toxoid.

作者信息

Hati R N, Mandal M, Hati A K

机构信息

Physiology Department, Indian Institute of Chemical Biology, Calcutta, India.

出版信息

Toxicon. 1990;28(8):895-902. doi: 10.1016/0041-0101(90)90019-4.

DOI:10.1016/0041-0101(90)90019-4

PMID:1964253

Abstract

Russell's viper venom detoxified by gamma (gamma)-radiation (100 kR or 200 kR) was used as a toxoid for active immunization of rabbits following a short or long schedule of immunization without any adjuvant. Effective neutralization of venom toxin by immune sera of rabbits was observed with both schedules. Sera of rabbits immunized with 100 kR irradiated venom toxoid (100 kR toxoid antisera) were more potent than 200 kR toxoid antisera. The presence of antibody in the immune sera was detected by immunoelectrophoresis. The effect of gamma-radiation on some enzymes and venom protein profiles was studied. Phosphodiesterase, protease and hyaluronidase were inhibited by radiation though phospholipase A activity remained unaffected. Radiation did not produce any gross change in the protein profile of crude viper venom. Phosphodiesterase and protease activities of viper venom were neutralized more effectively by 100 kR toxoid antisera in the short schedule than in the long schedule of immunization.

摘要

经γ（伽马）射线辐射（100千拉德或200千拉德）解毒的罗素蝰蛇毒，在不使用任何佐剂的情况下，按照短期或长期免疫程序，用作兔主动免疫的类毒素。两种免疫程序均观察到兔免疫血清对蛇毒毒素的有效中和作用。用100千拉德辐照的蛇毒类毒素免疫的兔血清（100千拉德类毒素抗血清）比200千拉德类毒素抗血清更有效。通过免疫电泳检测免疫血清中抗体的存在。研究了γ射线对某些酶和蛇毒蛋白质谱的影响。辐射抑制了磷酸二酯酶、蛋白酶和透明质酸酶，而磷脂酶A的活性未受影响。辐射未使粗制蝰蛇毒的蛋白质谱发生任何明显变化。在短期免疫程序中，100千拉德类毒素抗血清对蝰蛇毒磷酸二酯酶和蛋白酶活性的中和作用比对长期免疫程序更有效。