Zhang Xiao, Qin Wenjuan, Paice Michael G, Saddler John N
FPInnovations Paprican Division (PAPRICAN), 570 boul. St-Jean, Pointe-Claire, Quebec, Canada H9R 3J9.
Bioresour Technol. 2009 Dec;100(23):5890-7. doi: 10.1016/j.biortech.2009.06.082. Epub 2009 Jul 29.
The feasibility of using a laboratory peg mixer to carry out high consistency enzymatic hydrolysis of lignocellulosic substrates was investigated. Two hardwood substrates, unbleached hardwood pulp (UBHW) and organosolv pretreated poplar (OPP), were used in this study. Hydrolysis of UBHW and OPP at 20% substrate consistency led to a high glucose concentration in the final hydrolysate. For example, a 48 h enzymatic hydrolysis of OPP resulted in a hydrolysate with 158 g/L of glucose. This is the highest glucose concentration ever obtained from enzymatic hydrolysis of lignocellulosic substrates. Fermentation of UBHW and OPP hydrolysates with high glucose content led to high ethanol concentrations, 50.4 and 63.1 g/L, respectively after fermentation. Our results demonstrate that using common pulping equipment to carry out high consistency hydrolysis can overcome the rheological problems and greatly increase the sugar and ethanol concentrations after the hydrolysis and fermentation.
研究了使用实验室搅拌混合器对木质纤维素底物进行高浓度酶水解的可行性。本研究使用了两种阔叶木底物，即未漂阔叶木浆（UBHW）和有机溶剂预处理杨树（OPP）。在底物浓度为20%的情况下对UBHW和OPP进行水解，最终水解产物中葡萄糖浓度较高。例如，对OPP进行48小时的酶水解，得到的水解产物中葡萄糖含量为158克/升。这是从木质纤维素底物酶水解中获得的最高葡萄糖浓度。对葡萄糖含量高的UBHW和OPP水解产物进行发酵，发酵后乙醇浓度分别高达为50.4克/升和63.1克/升。我们的结果表明，使用普通制浆设备进行高浓度水解可以克服流变学问题，并大大提高水解和发酵后的糖浓度和乙醇浓度。
