Raghavendran Vijayendran, Nitsos Christos, Matsakas Leonidas, Rova Ulrika, Christakopoulos Paul, Olsson Lisbeth
Industrial Biotechnology Division, Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 412 96, Gothenburg, Sweden.
Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK.
AMB Express. 2018 Jul 10;8(1):114. doi: 10.1186/s13568-018-0643-y.
A shift towards a sustainable and green society is vital to reduce the negative effects of climate change associated with increased CO emissions. Lignocellulosic biomass is both renewable and abundant, but is recalcitrant to deconstruction. Among the methods of pretreatment available, organosolv (OS) delignifies cellulose efficiently, significantly improving its digestibility by enzymes. We have assessed the hydrolysability of the cellulose-rich solid fractions from OS-pretreated spruce and birch at 2% w/v loading (dry matter). Almost complete saccharification of birch was possible with 80 mg enzyme preparation/g (12 FPU/g), while the saccharification yield for spruce was only 70%, even when applying 60 FPU/g. As the cellulose content is enriched by OS, the yield of glucose was higher than in their steam-exploded counterparts. The hydrolysate was a transparent liquid due to the absence of phenolics and was also free from inhibitors. OS pretreatment holds potential for use in a large-scale, closed-loop biorefinery producing fuels from the cellulose fraction and platform chemicals from the hemicellulose and lignin fractions respectively.
向可持续和绿色社会转型对于减少与二氧化碳排放量增加相关的气候变化负面影响至关重要。木质纤维素生物质既可再生又丰富，但难以解构。在现有的预处理方法中，有机溶剂（OS）能有效地使纤维素脱木质素，显著提高其对酶的消化率。我们评估了在2% w/v负载量（干物质）下，经OS预处理的云杉和桦木中富含纤维素的固体部分的水解能力。使用80 mg酶制剂/g（12 FPU/g）时，桦木几乎可以完全糖化，而即使使用60 FPU/g，云杉的糖化产率也仅为70%。由于OS使纤维素含量增加，葡萄糖产率高于其蒸汽爆破处理后的对应物。水解产物是一种透明液体，因为没有酚类物质，也没有抑制剂。OS预处理在大规模闭环生物精炼厂中具有应用潜力，该生物精炼厂可分别从纤维素部分生产燃料，从半纤维素和木质素部分生产平台化学品。
