Kavalar Rajko, Pohar Marinsek Ziva, Jereb Berta, Cagran Branka, Golouh Rastko
Department of Pathology, University Medical Centre Maribor, Maribor, Slovenia.
Med Sci Monit. 2009 Aug;15(8):CR442-52.
The expressions of different markers have been immunohistochemically studied in various types of the Ewing's sarcoma family of tumors, especially for diagnostic purposes. However, little is known about their prognostic value in combination with the clinicopathological data of such patients.
MATERIAL/METHODS: This retrospective study investigated the immunohistochemical expressions of NSE, TdT, EMA, S-100, CK MNF116, p53, bcl-2, CD99, and CD117 on formalin-fixed paraffin-embedded material of 72 patients (age range: 2-59 years) with various types of Ewing's sarcoma family of tumors using the tissue microarray method. The immunohistochemical results were compared with clinicopathological features using survival analysis (Cox regression).
CD99 expression was detected in 90%, CD117 in 75.8%, bcl-2 in 70.1%, NSE in 66.6%, p53 in 66.6%, EMA in 26%, S-100 in 25.4%, TdT in 22.1%, and CK MNF116 in 10.2% of the cases. No immunoreactivity was observed in the normal tissue around the tumors.
The expressions of EMA (p=0.107), NSE (p=0.126), CD99 (p=0.14), TdT (p=0.198), bcl-2 (p=0.382), p53 (p=0.54), CD117 (p=0.612), S-100 (p=0.867), and CK MNF116 (p=0.934) had no statistically significant impact on survival. Patient age at the time of diagnosis (p=0.008) and the presence of tumor necrosis (p=0.033) were the only significant prognostic factors in this study. Tumor location was an insignificant prognostic factor (p=0.38).
已通过免疫组织化学方法研究了不同标志物在各种类型尤因肉瘤家族性肿瘤中的表达情况，尤其是用于诊断目的。然而，对于这些标志物与此类患者临床病理数据相结合的预后价值，人们了解甚少。
材料/方法：本回顾性研究采用组织芯片法，对72例（年龄范围：2至59岁）患有各种类型尤因肉瘤家族性肿瘤患者的福尔马林固定石蜡包埋材料进行了免疫组织化学检测，观察NSE、TdT、EMA、S-100、CK MNF116、p53、bcl-2、CD99和CD117的表达情况。使用生存分析（Cox回归）将免疫组织化学结果与临床病理特征进行比较。
在病例中，90%检测到CD99表达，75.8%检测到CD117表达，70.1%检测到bcl-2表达，66.6%检测到NSE表达，66.6%检测到p53表达，26%检测到EMA表达，25.4%检测到S-100表达，22.1%检测到TdT表达，10.2%检测到CK MNF116表达。在肿瘤周围的正常组织中未观察到免疫反应性。
EMA（p = 0.107）、NSE（p = 0.126）、CD99（p = 0.14）、TdT（p = 0.198）、bcl-2（p = 0.382）、p53（p = 0.54）、CD117（p = 0.612）、S-100（p = 0.867）和CK MNF116（p = 0.934）的表达对生存无统计学显著影响。本研究中，诊断时患者年龄（p = 0.008）和肿瘤坏死的存在（p = 0.033）是仅有的显著预后因素。肿瘤位置是一个无显著意义的预后因素（p = 0.38）。
