Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry, Morioka, Japan.
J Mater Sci Mater Med. 2010 Jan;21(1):267-75. doi: 10.1007/s10856-009-3834-x. Epub 2009 Jul 31.
This study was performed to microscopically observe and measure inflammatory cytokine production by human macrophages phagocytosing submicron titanium (Ti) particles. Observations with secondary electron microscopy (SEM), SEM/electron probe microanalysis (EPMA) and transmission electron microscopy (TEM) indicated that macrophages [phorbol-12-myristate-13-acetate (PMA)-differentiated THP-1 cells] at 24 h in culture actively phagocytosed and accumulated submicron Ti particles in intracellular phagosomes, in which refinement of Ti particles occurred. The macrophages were also cultured for 24 h in four media with and without submicron Ti particles and lipopolysaccharide (LPS; components of bacteria). Whilst neither stimulus reduced cell viability, submicron Ti particles and LPS activation independently and synergistically caused the macrophages to produce three inflammatory cytokines (TNF-alpha, IL-1beta and IL-6) at high levels in the culture supernatants. The inflammatory and osteolysis conditions caused by macrophages phagocytosing submicron Ti particles would be worsened by challenge with LPS in patients wearing Ti prostheses.
本研究旨在通过吞噬亚微米钛（Ti）颗粒的人巨噬细胞，在显微镜下观察和测量炎症细胞因子的产生。二次电子显微镜（SEM）、SEM/电子探针微分析（EPMA）和透射电子显微镜（TEM）的观察结果表明，培养 24 小时的巨噬细胞[佛波醇-12-肉豆蔻酸-13-醋酸酯（PMA）分化的 THP-1 细胞]可主动吞噬并在细胞内吞噬体中积累亚微米 Ti 颗粒，Ti 颗粒在此过程中被细化。巨噬细胞还在含和不含亚微米 Ti 颗粒及脂多糖（LPS；细菌成分）的四种培养基中培养 24 小时。虽然两种刺激物均未降低细胞活力，但亚微米 Ti 颗粒和 LPS 的单独和协同激活可导致巨噬细胞在培养上清液中高水平产生三种炎症细胞因子（TNF-α、IL-1β和 IL-6）。在佩戴钛假体的患者中，吞噬亚微米 Ti 颗粒的巨噬细胞引发的炎症和骨溶解状况可能因 LPS 的刺激而恶化。
