Schwarz Frank, Langer Maike, Hagena Tina, Hartig Brigitte, Sader Robert, Becker Jürgen
Department of Oral Surgery and Implantology, Carolinum, Goethe University, Frankfurt, Germany.
Department of Oral Surgery, Universitätsklinikum Düsseldorf, Düsseldorf, Germany.
Int J Implant Dent. 2019 Jul 9;5(1):25. doi: 10.1186/s40729-019-0178-2.
To assess the effects of differently sized titanium (Ti) and zirconia (Zr) particles on (1) the metabolic activity of osteosarcoma-derived osteoblasts (SaOs-2) and human gingival fibroblasts (HGF) and (2) the cytokine expression of monocytes (THP-1) METHODS: Ti (60-80 nm and 100 nm) and Zr (2 μm and 75 μm) particles were incubated with SaOs-2, HGF, and THP-1 cells. At days 0, 2, 4, and 7 and 0, 1, 2, and 4 (THP-1), the mitochondrial activity was assessed and enzyme-linked immunosorbent assays were used to determine interleukin (IL)-1 beta and IL-6 concentrations of stimulated THP-1 at day 1.
Ti60-80, Ti100, Zr2, and Zr75 particles were associated with gradual and significant within-group decreases in the viability of SaOs-2 and HGF cells. These effects were less pronounced in the Zr group. Similar to control cells, THP-1 did not reveal any significant increases in IL-1 beta and IL-6 concentrations. Viability of THP-1 was merely impaired in the presence of Ti100.
Ti and Zr particles had a detrimental effect on the viability of SaOs-2 and HGF, but no proinflammatory effect on THP-1.
评估不同尺寸的钛（Ti）和氧化锆（Zr）颗粒对（1）骨肉瘤来源的成骨细胞（SaOs-2）和人牙龈成纤维细胞（HGF）的代谢活性以及（2）单核细胞（THP-1）细胞因子表达的影响。
将Ti（60 - 80nm和100nm）和Zr（2μm和75μm）颗粒与SaOs-2、HGF和THP-1细胞共同孵育。在第0、2、4和7天以及第0、1、2和4天（THP-1）评估线粒体活性，并使用酶联免疫吸附测定法测定第1天受刺激的THP-1的白细胞介素（IL）-1β和IL-6浓度。
Ti60 - 80、Ti100、Zr2和Zr75颗粒与SaOs-2和HGF细胞活力在组内逐渐且显著降低有关。这些影响在Zr组中不太明显。与对照细胞相似，THP-1的IL-1β和IL-6浓度没有任何显著增加。仅在存在Ti100的情况下THP-1的活力受到损害。
Ti和Zr颗粒对SaOs-2和HGF的活力有不利影响，但对THP-1没有促炎作用。
