Shi Zhong-Feng, Li Ru-Liu, Chen Wei-Wen
Piwei Institute, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
Zhong Yao Cai. 2009 Apr;32(4):597-600.
To study the method of Atractylodes macrocephala monosaccharide composition (AMMC)'s extraction, purification and analysis.
To extract AMMC with water extraction method and purify it with neutro-alumina and resin adsorption method, to observe removal impurity effect of several methods and determine the technology parameters. To detect monosaccharide's content in AMMC with hydrochloric acid-ultraviolet spectrophotometric determination and Evaporative light scattering detector (ELSD).
After purificated with neutro-alumina, the yield and purity of AMMC were 23% - 25.5% and 56% - 60%, respectively. According to the static decoloring curve of D900, D201 and AB-8 resins, their decolorization effect's rank was D900 > AB-8 > D201. The yield of AMMC purificated with D900 ion exchange resin was above 17%, and the purity of monosaccharide was above 76%.
The effect of AMMC purificated with D900 ion exchange resin after water extration is much better, the ideal method for the quantitative analysis of monosaccharide is hydrochloric acid-ultraviolet spectrophotometric determination.
