大孔吸附树脂对鸡眼草总黄酮的富集与纯化研究

[Study on the enrichment and purification of total flavonoids in Microcos paniculata by macroporous adsorption resin].

作者信息

Li Kun-Ping, Pan Tian-Ling, Gao Chong-Kai, Li Wei-Min

机构信息

Guangdong Pharmaceutical University, Guangzhou 510006, China.

出版信息

Zhong Yao Cai. 2009 Apr;32(4):601-4.

PMID:19645249

Abstract

OBJECTIVE

To establish an optimum enrichment and purification process of total flavonoids in Microcos paniculata by macroporous resins.

METHODS

Five kinds of resins were compared and the best one was chosen. Then the parameters of the process were optimized by single factor tests, uniform design and statistical methods.

RESULTS

DI01 was selected for its excellent adsorption and desorption properties, 70% ethanol was found to be the best elution solution. As far as the yield was considered, the best result was based on the followings: feed rate-1.0 BV/h, elution flow rate-2.0 BV/h, sample concentration-7.88 mg/mL, eluting agent amount-2.0 BV, pH value 4.8; then the yield reached 90.18% and the purity was 54.37%. If the purity was considered, the best parameters wereas follows: feed rate-1.0 BV/h, elution flow rate-2.0 BV/h, sample concentration-2.0 mg/mL, eluting agent amount-2.8 BV, pH value 7.8; then the purity reached 61.77% and the yield was 80.25%.

CONCLUSION

The total flavonoids of Microcos paniculata can be effectively purificated and separated by D101 macroporous resin.

摘要

目的

建立用大孔树脂富集纯化布渣叶中总黄酮的最佳工艺。

方法

比较5种树脂，筛选出最佳树脂，再通过单因素试验、均匀设计及统计学方法对工艺参数进行优化。

结果

筛选出吸附与解吸性能良好的D101树脂，最佳洗脱剂为70%乙醇。从得率考虑，最佳工艺参数为：上样速度1.0 BV/h、洗脱流速2.0 BV/h、样品浓度7.88 mg/mL、洗脱剂用量2.0 BV、pH值4.8，此时得率达90.18%，纯度为54.37%；从纯度考虑，最佳工艺参数为：上样速度1.0 BV/h、洗脱流速2.0 BV/h、样品浓度2.0 mg/mL、洗脱剂用量2.8 BV、pH值7.8，此时纯度达61.77%，得率为80.25%。