Ozen Tevfik, Korkmaz Halil
Department of Chemistry, Faculty of Arts and Sciences, Giresun University, TR-28049 Giresun, Turkey.
Acta Pol Pharm. 2009 May-Jun;66(3):305-9.
The effects of hydroalcoholic (80% ethanol-20% water) extract of Urtica dioica L. on microsomal aniline 4-hydroxylase (A4H) were investigated in the liver of Swiss albino mice (8- 10-weeks-old) treated with two doses (50 and 100 mg/kg body weight, given orally for 14 days ). The activities of A4H showed a significant increase in the liver at both dose levels of extract treatment. The hydroalcoholic extract of Urtica dioica induced the activities of A4H that had been increased by treatment of metal ions (Mg2+ and Ca2+) and the mixture of cofactors (NADH and NADPH). At saturated concentration of cofactor, microsomal A4H exhibited significantly even higher activities in the presence of the mixture of cofactors than NADPH and NADH. Mg2+ and Ca2+ ions acted as stimulants in vitro. The present results suggest that the hydroalcoholic extract of Urtica dioica may have modalatory effect on aniline hydroxylase at least in part and enhance the activity of A4H adding metals ions and cofactors.
在瑞士白化小鼠（8 - 10周龄）的肝脏中，研究了荨麻水醇提取物（80%乙醇 - 20%水）对微粒体苯胺4 - 羟化酶（A4H）的影响。给予两剂量（50和100毫克/千克体重，口服14天）提取物处理小鼠。在提取物处理的两个剂量水平下，肝脏中A4H的活性均显著增加。荨麻水醇提取物诱导了因金属离子（Mg2 +和Ca2 +）及辅因子混合物（NADH和NADPH）处理而增加的A4H活性。在辅因子饱和浓度下，微粒体A4H在辅因子混合物存在时表现出比NADPH和NADH显著更高的活性。Mg2 +和Ca2 +离子在体外起刺激作用。目前的结果表明，荨麻水醇提取物可能至少部分地对苯胺羟化酶有调节作用，并通过添加金属离子和辅因子增强A4H的活性。
