Institute of Chemistry and Biochemistry, Free University Berlin, Takustrasse 3, D-14195 Berlin, Germany.
J Phys Chem B. 2009 Aug 27;113(34):11557-9. doi: 10.1021/jp905263x.
Single-stranded DNA oligonucleotides (33-mers) containing different numbers of guanines (n=1-4) were tethered to a gold surface and exposed to 1 eV electrons. The electrons induced DNA damage, which was analyzed with fluorescence and infrared spectroscopy methods. The damage was identified as strand breaks and found to correlate linearly with the number of guanines in the sequence. This sequence dependence indicates that the electron capture by the DNA bases plays an important role in the damage reaction mechanism.
单链 DNA 寡核苷酸（33 个碱基）含有不同数量的鸟嘌呤（n=1-4），被连接到金表面并暴露于 1 eV 电子下。电子诱导了 DNA 损伤，并用荧光和红外光谱方法进行了分析。损伤被鉴定为链断裂，并发现与序列中的鸟嘌呤数量呈线性相关。这种序列依赖性表明，DNA 碱基的电子俘获在损伤反应机制中起着重要作用。
