Fukushima F B, Malm C, Henry M, Gheller V A, Serakides R, Neves M M, Macedo S P, Figueiredo M S, Andrade M E J, Chaves M S, Silva M X, Rezende C M F, Melo E G
Department of Veterinary Clinics and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Reprod Domest Anim. 2010 Dec;45(6):1059-64. doi: 10.1111/j.1439-0531.2009.01493.x.
The aim of this study was to evaluate the distribution of frozen-thawed spermatozoa within the uterine lumen and oviducts following intrauterine laparoscopic deposition at two sites. Twelve bitches of unknown reproductive history were randomly distributed into two groups. Semen (3 ml containing 300 × 10(6) frozen-thawed spermatozoa) was infused at the uterine body (UB group) or at the cranial tip of the left uterine horn. A 22-G catheter was used to access the uterine lumen. Sperm cell distribution was evaluated after ovariohysterectomy performed 3 h after artificial insemination (AI). There was no difference between groups in mean time to perform AI. Spermatozoa were detected in all uterine segments, including the tip of both horns, but none was detected in the oviduct. The 22-G catheter facilitated deposition of semen in the uterine lumen, particularly at the UB site. Sperm cell distribution occurred evenly along both horns, independent of the site of semen deposition.
本研究的目的是评估在子宫内通过腹腔镜在两个部位进行精液沉积后，冻融精子在子宫腔和输卵管内的分布情况。将12只生殖史不明的母犬随机分为两组。在子宫体（UB组）或左子宫角的头端注入精液（3毫升，含3亿冻融精子）。使用22G导管进入子宫腔。在人工授精（AI）后3小时进行卵巢子宫切除术后评估精子细胞分布。两组在进行AI的平均时间上没有差异。在所有子宫段，包括两角的尖端，均检测到精子，但在输卵管中未检测到。22G导管有助于将精液沉积在子宫腔内，特别是在UB部位。精子细胞沿两角均匀分布，与精液沉积部位无关。
