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使用开放阅读框ESTs（ORESTES）对猪带绦虫囊尾蚴进行转录组分析。

Transcriptome analysis of Taenia solium cysticerci using Open Reading Frame ESTs (ORESTES).

作者信息

Almeida Carolina R, Stoco Patricia H, Wagner Glauber, Sincero Thaís Cm, Rotava Gianinna, Bayer-Santos Ethel, Rodrigues Juliana B, Sperandio Maísa M, Maia Antônio Am, Ojopi Elida Pb, Zaha Arnaldo, Ferreira Henrique B, Tyler Kevin M, Dávila Alberto Mr, Grisard Edmundo C, Dias-Neto Emmanuel

机构信息

Laboratórios de Protozoologia e de Bioinformática, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina (UFSC), Caixa postal 476, CEP 88040-970, Florianópolis, SC, Brazil.

出版信息

Parasit Vectors. 2009 Jul 31;2(1):35. doi: 10.1186/1756-3305-2-35.

DOI:10.1186/1756-3305-2-35
PMID:19646239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2731055/
Abstract

BACKGROUND

Human infection by the pork tapeworm Taenia solium affects more than 50 million people worldwide, particularly in underdeveloped and developing countries. Cysticercosis which arises from larval encystation can be life threatening and difficult to treat. Here, we investigate for the first time the transcriptome of the clinically relevant cysticerci larval form.

RESULTS

Using Expressed Sequence Tags (ESTs) produced by the ORESTES method, a total of 1,520 high quality ESTs were generated from 20 ORESTES cDNA mini-libraries and its analysis revealed fragments of genes with promising applications including 51 ESTs matching antigens previously described in other species, as well as 113 sequences representing proteins with potential extracellular localization, with obvious applications for immune-diagnosis or vaccine development.

CONCLUSION

The set of sequences described here will contribute to deciphering the expression profile of this important parasite and will be informative for the genome assembly and annotation, as well as for studies of intra- and inter-specific sequence variability. Genes of interest for developing new diagnostic and therapeutic tools are described and discussed.

摘要

背景

全球有超过5000万人感染猪带绦虫，尤其是在不发达国家和发展中国家。由幼虫包囊形成引起的囊尾蚴病可能危及生命且难以治疗。在此，我们首次研究了具有临床相关性的囊尾蚴幼虫形态的转录组。

结果

使用ORESTES方法产生的表达序列标签（ESTs），从20个ORESTES cDNA微型文库中总共产生了1520个高质量ESTs，其分析揭示了具有潜在应用价值的基因片段，包括51个与其他物种中先前描述的抗原匹配的ESTs，以及113个代表可能位于细胞外的蛋白质的序列，在免疫诊断或疫苗开发方面有明显应用。

结论

这里描述的一系列序列将有助于解读这种重要寄生虫的表达谱，对于基因组组装和注释以及种内和种间序列变异性研究也将提供信息。文中描述并讨论了开发新诊断和治疗工具所关注的基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d0e/2731055/66a57b9f5322/1756-3305-2-35-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d0e/2731055/aa178ecd7bf2/1756-3305-2-35-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d0e/2731055/66a57b9f5322/1756-3305-2-35-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d0e/2731055/aa178ecd7bf2/1756-3305-2-35-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d0e/2731055/66a57b9f5322/1756-3305-2-35-2.jpg

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