Centro de Investigacións Mariñas, Pedras de Corón, s/n. Apdo. 13, 36620 Vilanova de Arousa (Pontevedra), Spain.
Toxicon. 2010 Feb-Mar;55(2-3):221-6. doi: 10.1016/j.toxicon.2009.07.022. Epub 2009 Jul 29.
The subcellular distribution of okadaic acid, the main diarrhetic shellfish poisoning (DSP) toxin, in the cells of the digestive gland of the mussel Mytilus galloprovincialis was studied. By means of differential centrifugation, ultrafiltration and extraction with methanol, it was found that most okadaic acid was stored in the cytosol. Notwithstanding only a small proportion of the total toxin was found to be in free form, being most of it bound to a soluble cellular compound with a molecular mass which ranged from 30 to 300 kDa. A series of fractionations of samples digested with a protease, a lipase, and amylase suggested that the component to which okadaic acid is bound is a high density lipoprotein. A new fractionation after digestion with a protein lipase additionally supports the previous conclusion.
研究了麻痹性贝类毒素（DSP）的主要毒素——冈田酸在贻贝消化腺细胞中的亚细胞分布。通过差速离心、超滤和甲醇提取发现，大部分冈田酸储存在细胞质中。尽管只有一小部分总毒素以游离形式存在，大部分与分子量在 30 到 300 kDa 之间的可溶性细胞化合物结合。一系列用蛋白酶、脂肪酶和淀粉酶消化的样品的分级分离表明，冈田酸结合的成分是一种高密度脂蛋白。用蛋白脂肪酶消化后的新分级分离进一步支持了上述结论。
