Institute of Oceanography and Fisheries, Šet. I. Meštrovića 63, 21000 Split, Croatia.
Molecules. 2011 Jan 21;16(1):888-99. doi: 10.3390/molecules16010888.
Dinophysis spp. blooms and related shellfish toxicity events of diarrhetic shellfish poisoning (DSP) have been the most reported toxicity event through the Croatian National monitoring program. With the aim to characterize the DSP toxin profile in shellfish farmed in Croatia, for the first time a complete analysis of the toxin profile of Croatian mussels has been carried out using the LC-MS/MS technique. The obtained results showed okadaic acid (OA) as the main toxin contaminating Croatian mussels at that time. The maximum concentration of OA in shellfish tissue was recorded 12 days after the Dinophysis fortii bloom, thus suggesting that rapid growth of the toxin level in the shellfish occurred in the first week after the bloom while it was slower in the second week. Furthermore, the presence of only OA at concentrations which could endanger human health suggests D. fortii as the main organism responsible for the toxic event that occurred in Lim Bay. The presence of gymnodimine and spirolides in Croatian mussel has been detected for the first time, while the presence of yessotoxin and pectenotoxin-2 is confirmed.
双鞭甲藻属（Dinophysis spp.）水华和相关贝类麻痹性贝类毒素（DSP）中毒事件是通过克罗地亚国家监测计划报告的最常见的毒性事件。为了描述克罗地亚贝类养殖场的 DSP 毒素特征，首次使用 LC-MS/MS 技术对克罗地亚贻贝的毒素特征进行了全面分析。结果表明，当时贻贝中主要的毒素污染物是 okadaic acid（OA）。贝类组织中 OA 的最大浓度是在双鞭甲藻水华 12 天后记录到的，这表明毒素水平在水华后第一周迅速增长，而在第二周则较慢。此外，仅存在浓度足以危害人体健康的 OA，表明双鞭甲藻是发生在利姆湾的毒性事件的主要生物体。首次在克罗地亚贻贝中检测到了 gymnodimine 和 spirolides 的存在，同时确认了 yessotoxin 和 pectenotoxin-2 的存在。
