温敏性聚[（N-异丙基丙烯酰胺）-r-（（3-（甲基丙烯酰氨基）丙基）-二甲基（3-磺丙基）氢氧化铵）]薄膜上细胞黏附与脱离的控制

The control of cell adhesion and detachment on thin films of thermoresponsive poly[(N-isopropylacrylamide)-r-((3-(methacryloylamino)propyl)-dimethyl(3-sulfopropyl)ammonium hydroxide)].

作者信息

Kong Bokyung, Choi Joon S, Jeon Saewha, Choi Insung S

机构信息

Department of Chemistry, KAIST, Daejeon 305-701, South Korea.

出版信息

Biomaterials. 2009 Oct;30(29):5514-22. doi: 10.1016/j.biomaterials.2009.07.016. Epub 2009 Jul 30.

DOI:10.1016/j.biomaterials.2009.07.016

PMID:19646752

Abstract

This paper describes the formation of poly(N-isopropylacrylamide) (PNIPAAm) and poly[(N-isopropylacrylamide)-r-((3-(methacryloylamino)propyl)-dimethyl(3-sulfopropyl)ammonium hydroxide)] (P(NIPAAm-r-MPDSAH)) films on a glass surface via surface-initiated, atom transfer radical polymerization as a cell-culture platform. The films of PNIPAAm with various thicknesses and of P(NIPAAm-r-MPDSAH) with various ratios of NIPAAm and MPDSAH are formed to investigate the behaviors of cell adhesion and detachment. In the case of the PNIPAAm-grafted glass surfaces, the optimal film thickness, achieving the effective control of both cell adhesion and detachment, is estimated to be 11-13 nm for NIH 3T3 fibroblast cells. The adhesion and detachment behaviors of NIH 3T3 fibroblast cells are further tuned by incorporating the hydrophilic and non-biofouling MPDSAH moiety into the PNIPAAm system. The cell adhesion and detachment are controlled best, when the ratio of NIPAAm and MPDSAH is 75:1 (NIPAAm:MPDSAH).

摘要

本文描述了通过表面引发的原子转移自由基聚合反应在玻璃表面形成聚（N-异丙基丙烯酰胺）（PNIPAAm）和聚[（N-异丙基丙烯酰胺）-r-（（3-（甲基丙烯酰氨基）丙基）-二甲基（3-磺丙基）氢氧化铵）]（P（NIPAAm-r-MPDSAH））薄膜，作为细胞培养平台。制备了不同厚度的PNIPAAm薄膜以及不同NIPAAm与MPDSAH比例的P（NIPAAm-r-MPDSAH）薄膜，以研究细胞黏附与脱离行为。在PNIPAAm接枝的玻璃表面情况下，对于NIH 3T3成纤维细胞，实现对细胞黏附与脱离有效控制的最佳薄膜厚度估计为11 - 13 nm。通过将亲水性且无生物污染的MPDSAH部分引入PNIPAAm体系，进一步调节NIH 3T3成纤维细胞的黏附与脱离行为。当NIPAAm与MPDSAH的比例为75:1（NIPAAm:MPDSAH）时，细胞黏附与脱离的控制效果最佳。