State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, College of Materials Science & Engineering, Beijing University of Chemical Technology, Beijing, China.
Colloids Surf B Biointerfaces. 2011 Jun 15;85(1):40-7. doi: 10.1016/j.colsurfb.2010.09.027. Epub 2010 Oct 8.
Thermoresponsive poly(N-isopropylacrylamide) (P(NIPAAm))-grafted polycaprolactone (PCL) films with a suitable amount of immobilized cell-adhesive collagen were prepared to improve cell adhesion and proliferation above the lower critical solution temperature (LCST, 32°C) of P(NIPAAm) without destroying cell detachment properties at lower temperatures. Covalently tethered P(NIPAAm) brushes on PCL film surfaces were first prepared via surface-initiated atom transfer radical polymerization (ATRP). The alkyl bromide end groups of the grafted P(NIPAAm) brushes were used in nucleophilic substitution reactions for the direct coupling of collagen to produce the collagen-immobilized thermoresponsive PCL surface. At 37°C, the cell attachments on the collagen-immobilized thermoresponsive PCL surface were enhanced substantially. The attached cells could be recovered simply by lowering culture temperature. The P(NIPAAm)-grafted PCL films with immobilized collagen are potentially useful as adhesion modifiers for advanced cell culture and tissue engineering applications.
制备了具有适量固定化细胞黏附性胶原的温敏性聚（N-异丙基丙烯酰胺）（P（NIPAAm））接枝聚己内酯（PCL）薄膜，以提高细胞黏附性和增殖性，其方式是在 P（NIPAAm）的低临界溶液温度（LCST，32°C）以上，而不会在较低温度下破坏细胞脱离性能。首先通过表面引发原子转移自由基聚合（ATRP）在 PCL 薄膜表面制备了共价键接枝的 P（NIPAAm）刷。接枝的 P（NIPAAm）刷的烷基溴末端基团用于亲核取代反应，将胶原直接偶联到产生胶原固定的温敏性 PCL 表面。在 37°C 时，胶原固定的温敏性 PCL 表面上的细胞附着得到了很大的提高。通过降低培养温度，可以简单地回收附着的细胞。具有固定化胶原的 P（NIPAAm）接枝 PCL 薄膜有望作为高级细胞培养和组织工程应用的黏附修饰剂。
