Li I-Hsun, Huang Wen-Sheng, Yeh Chin-Bin, Liao Mei-Hsiu, Chen Chia-Chieh, Shen Lie-Hang, Liu Jiang-Chuan, Ma Kuo-Hsing
Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei 114, Taiwan.
Nucl Med Biol. 2009 Aug;36(6):605-11. doi: 10.1016/j.nucmedbio.2009.03.003.
Parkinson's disease (PD) affects both dopaminergic and serotonergic systems. In this study, we simultaneously evaluated dopamine and serotonin transporters in primates using dual-isotope single-photon emission computed tomography (SPECT) imaging and compared the results with traditional single-isotope imaging.
Four healthy and one 6-OHDA-induced PD monkeys were used for this study. SPECT was performed over 4 h after individual or simultaneous injection of [(99m)Tc]TRODAT-1 (a dopamine transporter imaging agent) and [(123)I]ADAM (a serotonin transporter imaging agent).
The results showed that the image quality and uptake ratios in different brain regions were comparable between single- and dual-isotope studies. The striatal [(99m)Tc]TRODAT-1 uptake in the PD monkey was markedly lower than that in normal monkeys. The uptake of [(123)I]ADAM in the midbrain of the PD monkey was comparable to that in the normal monkeys, but there were decreased uptakes in the thalamus and striatum of the PD monkey.
Our results suggest that dual-isotope SPECT using [(99m)Tc]TRODAT-1 and [(123)I]ADAM can simultaneously evaluate changes in dopaminergic and serotonergic systems in a PD model.
帕金森病（PD）会影响多巴胺能和5-羟色胺能系统。在本研究中，我们使用双同位素单光子发射计算机断层扫描（SPECT）成像技术同时评估了灵长类动物中的多巴胺和5-羟色胺转运体，并将结果与传统的单同位素成像进行了比较。
本研究使用了4只健康猴子和1只6-羟基多巴胺（6-OHDA）诱导的帕金森病猴子。在单独或同时注射[(99m)Tc]TRODAT-1（一种多巴胺转运体显像剂）和[(123)I]ADAM（一种5-羟色胺转运体显像剂）后4小时进行SPECT检查。
结果显示，单同位素和双同位素研究中不同脑区的图像质量和摄取率相当。帕金森病猴子纹状体中[(99m)Tc]TRODAT-1的摄取明显低于正常猴子。帕金森病猴子中脑[(123)I]ADAM的摄取与正常猴子相当，但帕金森病猴子丘脑和纹状体的摄取减少。
我们的结果表明，使用[(99m)Tc]TRODAT-1和[(123)I]ADAM的双同位素SPECT能够在帕金森病模型中同时评估多巴胺能和5-羟色胺能系统的变化。
