非人灵长类动物中同时进行的[99mTc]TRODAT-1和[123I]ADAM脑单光子发射计算机断层扫描
Simultaneous [99mTc]TRODAT-1 and [123I]ADAM brain SPECT in nonhuman primates.
作者信息
Ma Kuo-Hsing, Lee Jong-Kang, Huang San-Yuan, Yeh Chin-Bin, Shen Yi-Chun, Shen Lie-Hang, Chen Chia-Chieh, Liu Ren-Shyan, Liu Jiang-Chuan, Huang Wen-Sheng
机构信息
Department of Biology and Anatomy, National Defense Medical Center, Taipei, Taiwan.
出版信息
Mol Imaging Biol. 2009 Jul-Aug;11(4):253-62. doi: 10.1007/s11307-009-0197-0. Epub 2009 Feb 19.
PURPOSE
This study examined the feasibility of simultaneous dopamine and serotonin transporter imaging using [(123)I]ADAM and [(99m)Tc]TRODAT-1 single photon emission computed tomography (SPECT).
PROCEDURES
Simultaneous [(123)I]ADAM (185 MBq) and [(99m)Tc]TRODAT-1 (740 MBq) SPECT was performed in three age-matched female Formosan rock monkeys. An asymmetric energy window was used for dual, and symmetric energy windows were used for single-isotope imaging. Oral fluoxetine (20 mg) and intravenous methylphenidate HCl (1 mg/kg) were given 24 h and 10 min, respectively, before dual-isotope SPECT to test imaging specificities of [(123)I]ADAM and [(99m)Tc]TRODAT-1.
RESULTS
Comparable image quality and uptake ratios between dual- and single-isotope SPECT scans were found. Dual-isotope SPECT in fluoxetine-pretreated monkeys showed decreased uptake of [(123)I]-ADAM, but not of [(99m)Tc]TRODAT-1. Dual-isotope SPECT in methylphenidate-pretreated monkeys showed decreased [(99m)Tc]TRODAT-1 uptake without affecting [(123)I]-ADAM uptake.
CONCLUSION
Simultaneous [(123)I]-ADAM and [(99m)Tc]TRODAT-1 SPECT appears promising in nonhuman primates and may provide a suitable preclinical model with further clinical implications.
目的
本研究使用[（123）I]ADAM和[（99m）Tc]TRODAT-1单光子发射计算机断层扫描（SPECT）检测同时进行多巴胺和5-羟色胺转运体成像的可行性。
程序
对三只年龄匹配的雌性台湾猕猴进行了同时的[（123）I]ADAM（185MBq）和[（99m）Tc]TRODAT-1（740MBq）SPECT检查。采用不对称能量窗进行双同位素成像，采用对称能量窗进行单同位素成像。在双同位素SPECT检查前24小时和10分钟分别口服氟西汀（20mg）和静脉注射盐酸哌甲酯（1mg/kg），以检测[（123）I]ADAM和[（99m）Tc]TRODAT-1的成像特异性。
结果
双同位素和单同位素SPECT扫描之间的图像质量和摄取率相当。在氟西汀预处理的猕猴中，双同位素SPECT显示[（123）I]-ADAM摄取减少，但[（99m）Tc]TRODAT-1摄取未减少。在哌甲酯预处理的猕猴中，双同位素SPECT显示[（99m）Tc]TRODAT-1摄取减少，而不影响[（123）I]-ADAM摄取。
结论
同时进行[（123）I]-ADAM和[（99m）Tc]TRODAT-1 SPECT在非人灵长类动物中似乎很有前景，可能为进一步的临床应用提供合适的临床前模型。
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