Godovikova T S, Zarytova V F, Lokhov S G, Mal'tseva T V, Sergeev D S
Bioorg Khim. 1990 Oct;16(10):1369-78.
Daunomycin derivatives of pT(DT) and oligodeoxynucleotides were synthesized using reactive zwitter-ionic 4-N,N-dimethylaminopyridine derivatives of the terminal phosphate group. Daunomycin oligodeoxynucleotide analogues form more stable complementary complexes than the corresponding non-modified oligonucleotides. Both one- and two-dimensional (2D NOESY and 2D COSY) NMR spectra of DT were recorded and the proton signals assigned. From the detected cross-relaxation between H6 of thymidine and H1', H2', H2" of the carbohydrate residue of daunomycin it was concluded that, in DMSO, the DT molecule has a rather stable conformation, apparently due to the stacking interaction between the mononucleotide and daunomycin residues.
使用末端磷酸基团的反应性两性离子4-N，N-二甲基氨基吡啶衍生物合成了pT(DT)和寡聚脱氧核苷酸的柔红霉素衍生物。柔红霉素寡聚脱氧核苷酸类似物比相应的未修饰寡核苷酸形成更稳定的互补复合物。记录了DT的一维和二维（二维NOESY和二维COSY）核磁共振谱并对质子信号进行了归属。从胸腺嘧啶核苷的H6与柔红霉素碳水化合物残基的H1'、H2'、H2"之间检测到的交叉弛豫得出结论，在二甲亚砜中，DT分子具有相当稳定的构象，这显然是由于单核苷酸和柔红霉素残基之间的堆积相互作用。
