Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, Abymes 97183 Guadeloupe.
Infect Genet Evol. 2009 Dec;9(6):1400-5. doi: 10.1016/j.meegid.2009.07.007. Epub 2009 Aug 6.
Here, we present results of the first study of the Mycobacterium tuberculosis genotypes circulating in Kyrgyzstan. We focused on the incarcerated population known to be at high-risk for tuberculosis (TB) and with a significant impact on TB incidence in the general population. Beijing genotype was detected in 42 of 56 M. tuberculosis sputum-extracted DNA samples from newly-diagnosed adult pulmonary TB patients. RIF and INH resistance was genotypically detected in 28% and 55% samples; 13 of 15 MDR strains belonged to Beijing genotype. 12-locus MIRU-VNTR typing showed 8 of 56 samples to be mixed cases; 7 of them contained a Beijing strain. MIRU analysis demonstrated a high homogeneity of the studied collection (HGI=0.66) while 28 of 56 strains had a profile 223325153533 corresponding to Beijing/M2 subtype highly prevalent in different Russian settings. Three hypervariable loci, QUB-3232, VNTR-3820 and VNTR-4120, permitted to further subdivide 28 Beijing/M2 strains into 11 subtypes shared by 1 to 9 strains. To conclude, all markers taken together, the penitentiary population of M. tuberculosis in Kyrgyzstan exhibited a strong genetic affinity to Russia and a weak relatedness to East Asia.
在这里，我们呈现了在吉尔吉斯斯坦流行的结核分枝杆菌基因型的第一项研究结果。我们专注于被认为处于结核病（TB）高风险的被监禁人群，他们对普通人群的结核病发病率有重大影响。在新诊断的成人肺结核患者的 56 份结核分枝杆菌痰提取 DNA 样本中，检测到 42 份北京基因型。28%和 55%的样本中检测到利福平（RIF）和异烟肼（INH）耐药基因型；15 株耐多药菌株中有 13 株属于北京基因型。12 个基因座 MIRU-VNTR 分型显示 56 份样本中有 8 份为混合病例；其中 7 份含有北京株。MIRU 分析表明，所研究的样本高度同源（HGI=0.66），而 56 株中有 28 株的 223325153533 图谱与在不同俄罗斯地区高度流行的北京/M2 亚型相对应。三个高变区 QUB-3232、VNTR-3820 和 VNTR-4120 可进一步将 28 株北京/M2 株分为 11 个亚型，1 至 9 株之间存在共享。总之，所有标记综合来看，吉尔吉斯斯坦的监狱结核分枝杆菌人群与俄罗斯具有很强的遗传亲和力，与东亚的相关性较弱。
