Kovalev S Y, Kamaev E Y, Kravchenko M A, Kurepina N E, Skorniakov S N
Ural State Aleksei Maksimovich Gorky University, Ekaterinburg, Russian Federation.
Int J Tuberc Lung Dis. 2005 Jul;9(7):746-52.
The Ural region in Russia is one of the areas most affected by a high incidence of tuberculosis (TB). Molecular epidemiological studies able to trace Mycobacterium tuberculosis transmission are of particular significance.
To characterize the population of M. tuberculosis strains circulating in the Ural region, to detect the predominant genotypes and to evaluate their phylogenetic relationship and epidemiological significance.
Ninety-two M. tuberculosis clinical samples originating from the Ural region were genotyped using the MIRU-VNTR method.
Two major phylogenetically distinct groups of isolates were identified: the W-Beijing family (54.3%) and a previously unreported cluster, named the Ural group (15.2%). Forty-seven different MIRU profiles were identified, including 38 unique (41.3%) and 54 isolates grouped into nine clusters (from 2 to 28 isolates in each cluster). Genetic diversity within the clusters was shown by additional sub-typing of M. tuberculosis isolates in nine additional QUB-VNTR loci.
W-Beijing family isolates are associated with multiresistance to anti-tuberculosis drugs. It is possible that the strains of this family play a significant role in the spread of multidrug-resistant TB over the Ural region.
俄罗斯的乌拉尔地区是结核病（TB）高发病率影响最严重的地区之一。能够追踪结核分枝杆菌传播的分子流行病学研究具有特别重要的意义。
对乌拉尔地区流行的结核分枝杆菌菌株群体进行特征分析，检测主要基因型，并评估它们的系统发育关系和流行病学意义。
采用MIRU-VNTR方法对来自乌拉尔地区的92份结核分枝杆菌临床样本进行基因分型。
鉴定出两个主要的系统发育不同的分离株群体：W-北京家族（54.3%）和一个以前未报告的聚类，命名为乌拉尔组（15.2%）。鉴定出47种不同的MIRU图谱，包括38种独特的图谱（41.3%）和54株分离株，分为9个聚类（每个聚类中有2至28株分离株）。通过在另外9个QUB-VNTR位点对结核分枝杆菌分离株进行额外的亚型分析，显示了聚类内的遗传多样性。
W-北京家族分离株与耐多药结核病相关。该家族的菌株可能在耐多药结核病在乌拉尔地区的传播中发挥重要作用。
