Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022, Vladivostok, Russia.
Department of Microbiology, Chungnam National University, 220 Gung-dong, Yusong, Daejon 305-764, Republic of Korea.
Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):214-218. doi: 10.1099/ijs.0.012286-0. Epub 2009 Jul 31.
The taxonomic status of two novel heterotrophic, Gram-negative, gliding and yellow pigmented bacterial strains was established in this study. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the strains formed a distinct lineage within the genus Maribacter, a member of the family Flavobacteriaceae, with sequence similarities of 96.3-98.5 % to recognized species of the genus Maribacter. The maximum growth temperature of the strains was 35 degrees C and they required NaCl or seawater for growth. They hydrolysed aesculin and gelatin, reduced nitrates to nitrites and produced acid from carbohydrates. The DNA G+C contents of strains KMM 6025 and KMM 6046(T) were 36-37 mol%. On the basis of phenotypic, genotypic and phylogenetic characteristics, it is suggested that the new isolates represent a novel species of the genus Maribacter, for which the name Maribacter stanieri sp. nov. is proposed. The type strain is KMM 6046(T) (=KCTC 22023(T)=LMG 22581(T)).
在本研究中，确定了两株新型异养、革兰氏阴性、滑行和黄色色素细菌菌株的分类地位。基于 16S rRNA 基因测序的系统发育分析表明，这些菌株在黄杆菌科的海杆菌属内形成了一个独特的谱系，与海杆菌属的公认种的序列相似性为 96.3-98.5%。这些菌株的最大生长温度为 35°C，它们需要 NaCl 或海水才能生长。它们水解马栗树皮苷和明胶，将硝酸盐还原为亚硝酸盐，并从碳水化合物中产生酸。菌株 KMM 6025 和 KMM 6046(T)的 DNA G+C 含量为 36-37mol%。基于表型、基因型和系统发育特征，建议新分离株代表海杆菌属的一个新种，命名为斯坦氏海杆菌（Maribacter stanieri）sp. nov.。模式菌株为 KMM 6046(T)（=KCTC 22023(T)=LMG 22581(T)）。
