Lee Dong Wan, Lee Hanbyul, Kwon Bong-Oh, Khim Jong Seong, Yim Un Hyuk, Park Hongjae, Park Byeonghyeok, Choi In-Geol, Kim Beom Seok, Kim Jae-Jin
1Division of Environmental Science and Ecological Engineering, College of Life Science and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
2School of Earth and Environmental Science and Research Institute of Oceanography, Seoul National University, Seoul, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Nov;68(11):3471-3478. doi: 10.1099/ijsem.0.003011. Epub 2018 Sep 17.
A novel Gram-stain-negative, moderately halophilic and aerobic bacterium, designated strain SDRB-Phe2, was isolated from coastal sediment of the yellow sea in Sindu-ri, Republic of Korea. Cells were oxidase-positive, catalase-positive, rod-shaped and surrounded by a capsule with gliding motility. Colonies were yellow-coloured, circular, pulvinate with entire margins. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SDRB-Phe2 formed a distinct lineage within the genus Maribacter of the family Flavobacteriaceae. Stain SDRB-Phe2 exhibited 16S rRNA gene sequence similarity values of 97.1-98.9 % to the type strains of Maribacterstanieri, Maribacterspongiicola, Maribacter forsetii, Maribacter dokdonensis, Maribacter aquivivus, Maribactercaenipelagi, Maribacterlitorisediminis, Maribactersedimenticola, Maribacterulvicola, Maribacter confluentis and Maribacter orientalis, and of 94.8-96.7 % to the type strains of the other species of the genus Maribacter. Strain SDRB-Phe2 contained MK-6 as the predominant respiratory quinone and iso-C15 : 1 G, iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as the major fatty acids. The polar lipids of strain SDRB-Phe2 were phosphatidylethanolamine, one unidentified amino lipid and two unidentified lipids. The DNA G+C content was 36.2 mol%. DNA-DNA relatedness values of strain SDRB-Phe2 to the type strains of the 11 phylogenetically related species of the genus Maribacter were 21.9-38.6 %. On the basis of the phenotypic features, phylogenetic and DNA-DNA hybridization analyses presented here, strain SDRB-Phe2 (=JCM 32373=KCTC 62273=DSM 106042) represents a novel species of the genus Maribacter, for which the name Maribacterlitoralis sp. nov. is proposed.
从韩国新斗里黄海沿岸沉积物中分离出一株新型革兰氏阴性、中度嗜盐需氧细菌，命名为菌株SDRB-Phe2。细胞氧化酶阳性、过氧化氢酶阳性、杆状，被荚膜包围，具有滑行运动能力。菌落呈黄色、圆形、凸起，边缘整齐。基于16S rRNA基因序列的系统发育分析表明，菌株SDRB-Phe2在黄杆菌科海杆菌属内形成了一个独特的分支。菌株SDRB-Phe2与斯坦尼尔海杆菌、海绵海杆菌、福塞蒂海杆菌、独岛海杆菌、食水海杆菌、深海海杆菌、潮间带海杆菌、沉积物海杆菌、泥炭海杆菌、融合海杆菌和东方海杆菌模式菌株的16S rRNA基因序列相似性值为97.1-98.9%，与海杆菌属其他物种模式菌株的相似性值为94.8-96.7%。菌株SDRB-Phe2以MK-6作为主要呼吸醌，以异-C15:1 G、异-C15:0和特征性总和3（C16:1ω7c和/或C16:1ω6c）作为主要脂肪酸。菌株SDRB-Phe2的极性脂质为磷脂酰乙醇胺、一种未鉴定的氨基脂质和两种未鉴定的脂质。DNA G+C含量为36.2 mol%。菌株SDRB-Phe2与海杆菌属11个系统发育相关物种模式菌株的DNA-DNA相关性值为21.9-38.6%。基于此处呈现的表型特征、系统发育和DNA-DNA杂交分析，菌株SDRB-Phe2（=JCM 32373=KCTC 62273=DSM 106042）代表海杆菌属的一个新物种，为此提出新名称潮间带海杆菌（Maribacterlitoralis sp. nov.）。
