Pankajakshan Praveen, Zhang Bo, Blanc-Féraud Laure, Kam Zvi, Olivo-Marin Jean-Christophe, Zerubia Josiane
Ariana Project-team, INRIA/CNRS, 2004 Route des Lucioles, BP 93, 06902 Sophia-Antipolis Cedex, France.
Appl Opt. 2009 Aug 1;48(22):4437-48. doi: 10.1364/ao.48.004437.
We propose an alternate minimization algorithm for estimating the point-spread function (PSF) of a confocal laser scanning microscope and the specimen fluorescence distribution. A three-dimensional separable Gaussian model is used to restrict the PSF solution space and a constraint on the specimen is used so as to favor the stabilization and convergence of the algorithm. The results obtained from the simulation show that the PSF can be estimated to a high degree of accuracy, and those on real data show better deconvolution as compared to a full theoretical PSF model.
我们提出了一种交替最小化算法，用于估计共聚焦激光扫描显微镜的点扩散函数（PSF）和样本荧光分布。使用三维可分离高斯模型来限制PSF解空间，并对样本施加约束，以促进算法的稳定性和收敛性。模拟结果表明，PSF能够被高精度地估计，而实际数据结果显示，与完整的理论PSF模型相比，去卷积效果更好。
