Zhao Wang, Wang Qiu-xu, Liu Wei-xian
Department of Stomatology, Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China.
Shanghai Kou Qiang Yi Xue. 2009 Jun;18(3):307-12.
To investigate the effect of platelet-rich plasma (PRP) and osteoinduction active material(OAM) compounded with PRP in the repair of bone defects of peri-implant and evaluate the feasibility of PRP as a material for repair of peri-implant bone defects.
Four Beagle dogs underwent extraction of the mandibular first, second and fourth premolars to create edentulous regions. Three months later, titanium implants were inserted and bone defects around implants were created. The bone defects were repaired with PRP/OAM compounds in experimental group A, repaired with PRP/ tricalcium phosphate in experimental group B, and repaired with tricalcium phosphate in the control group. The animals were sacrificed at the end of 8-week and 16-week, respectively. The specimens were observed with histological examination and energy disperse analysis of Ca(2+). The data were analyzed with SPSS13.0 software package for one-way ANOVA.
At 8-week, in experimental group A, some parts of implants were directly contacted with new bone. Some new bones formed in the experimental group B. There was fibrous connection between the bone and implant in the control group. At 16-week, there was mature Haversian system in the experimental group A. There was good osseointegration between the bone and implant in the experimental groups but only was fibrous connection in the control group. At 8-and 16-week, there was significant difference between there groups in the percentage of Ca(2+) (P<0.05).
PRP and PRP/OAM composite can effectively accelerate the regeneration of peri-implant bone defects and improve the osseointegration in the interface between the implant and bone.
探讨富血小板血浆（PRP）及PRP复合骨诱导活性材料（OAM）对种植体周围骨缺损修复的影响，评估PRP作为种植体周围骨缺损修复材料的可行性。
4只比格犬拔除下颌第一、二、四前磨牙，形成无牙区。3个月后植入钛种植体，并在种植体周围制造骨缺损。A实验组用PRP/OAM复合物修复骨缺损，B实验组用PRP/磷酸三钙修复，对照组用磷酸三钙修复。分别在8周和16周结束时处死动物。对标本进行组织学检查及Ca(2+)能谱分析。数据用SPSS13.0软件包进行单因素方差分析。
8周时，A实验组部分种植体与新骨直接接触，B实验组有一些新骨形成，对照组骨与种植体间为纤维连接。16周时，A实验组有成熟的哈弗斯系统。实验组骨与种植体间骨结合良好，而对照组仅为纤维连接。8周和16周时，三组间Ca(2+)百分比有显著差异（P<0.05）。
PRP及PRP/OAM复合物能有效促进种植体周围骨缺损的再生，提高种植体与骨界面的骨结合。
