Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, 214122, Jiangsu, China.
Appl Biochem Biotechnol. 2010 Apr;160(8):2486-97. doi: 10.1007/s12010-009-8708-7. Epub 2009 Aug 1.
epsilon-Poly-L-lysine (epsilon-PL), a natural biomacromolecule having a broad spectrum of antibacterial activity, was grafted on the wool fiber via the acyl transfer reaction catalyzed by microbial transglutaminase (mTGase) to develop a new strategy for antibacterial functionalization of proteinous materials. The effects of the concentrations of epsilon-PLs and mTGases on the graft yields were investigated. A coating of epsilon-PL that almost completely covered the scale profile on the wool surface was visualized by scanning electron microscopy (SEM) and further demonstrated in terms of Allwörden's reaction characteristic of wool. Identifiable differences in lysine content and color depth among the stained wool samples reveal the changes in the surface composition and polarity caused by the incorporation of epsilon-PL onto the wool substrate, respectively. The ratio of bacteriostasis to Escherichia coli of the wool fabric grafting epsilon-PL reached 96.6 %, indicating an excellent antibacterial effect. The application of epsilon-PL and corresponding mTGase-catalyzed grafting reaction would provide a worthwhile reference for antibacterial functionalization of proteinous materials in various forms.
ε-聚赖氨酸（ε-PL）是一种具有广谱抗菌活性的天然生物大分子，通过微生物谷氨酰胺转移酶（mTGase）催化的酰基转移反应接枝到羊毛纤维上，为蛋白质材料的抗菌功能化开发了一种新策略。研究了ε-PLs 和 mTGases 的浓度对接枝率的影响。通过扫描电子显微镜（SEM）可以观察到ε-PL 几乎完全覆盖了羊毛表面鳞片的形貌，并且可以根据羊毛的 Allwörden 反应特征进一步证明。经过染色的羊毛样品中赖氨酸含量和颜色深度的可识别差异分别揭示了ε-PL 接枝到羊毛基质上引起的表面组成和极性的变化。接枝 ε-PL 的羊毛织物的抑菌率对大肠杆菌达到 96.6%，表明具有优异的抗菌效果。ε-PL 的应用及其相应的 mTGase 催化接枝反应将为各种形式的蛋白质材料的抗菌功能化提供有价值的参考。
