Wang Qiang, Fan Xuerong, Hu Yingjun, Yuan Jiugang, Cui Li, Wang Ping
Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, Jiangsu, China.
Bioprocess Biosyst Eng. 2009 Aug;32(5):633-9. doi: 10.1007/s00449-008-0286-5. Epub 2008 Dec 12.
Greater attention has been given to enzymatic processes of textiles as effective alternatives to conventional chemical treatments because of the non-toxic and eco-friendly characteristics of enzymes as well as the increasingly important requirement for reducing pollution in textile production. A new functionalization method for wool fabrics based on immobilization of lysozymes was investigated in this paper. Wool fabric was first activated with glutaraldehyde, and then employed to covalently immobilize lysozymes. Main immobilization parameters were optimized in terms of the activity of immobilized enzyme. A high activity of the immobilized enzyme was obtained when the fabric was activated at 25 degrees C for 6 h in a bath containing with 0.2% of glutaraldehyde followed by the immobilization at 4 degrees C and pH 7.0 for 6 h with 5 g l(-1) lysozyme. The scanning electron microscopy and staining tests based on modified Coomassie protein assay (Bradford method) revealed that the lysozyme was fixed covalently on the wool fabric. Wool fabrics immobilizing lysozymes presented a higher ratio of bacteriostasis to Staphylococcus aureus. The durability of antibacterial wool was assessed and the lysozyme immobilized on wool fabric retained ca. 43% of its activity after five cycles of use when taking the activity of the immobilized lysozyme before using as reference.
由于酶具有无毒和环保的特性，以及纺织生产中对减少污染的要求日益重要，因此人们越来越关注酶法处理纺织品，将其作为传统化学处理的有效替代方法。本文研究了一种基于溶菌酶固定化的羊毛织物功能化新方法。羊毛织物先用戊二醛活化，然后用于共价固定溶菌酶。根据固定化酶的活性对主要固定化参数进行了优化。当织物在含有0.2%戊二醛的浴中于25℃活化6小时，然后在4℃和pH 7.0下用5 g l(-1)溶菌酶固定6小时时，获得了高活性的固定化酶。基于改良考马斯亮蓝蛋白质测定法（Bradford法）的扫描电子显微镜和染色试验表明，溶菌酶共价固定在羊毛织物上。固定有溶菌酶的羊毛织物对金黄色葡萄球菌具有更高的抑菌率。评估了抗菌羊毛的耐久性，以使用前固定化溶菌酶的活性为参考时，固定在羊毛织物上的溶菌酶在使用五个循环后保留了约43%的活性。
