Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
J Proteome Res. 2009 Oct;8(10):4479-86. doi: 10.1021/pr9002596.
The Multiple T-maze (MTM) and the Barnes maze (BM) are land mazes used for the evaluation of spatial memory. The observation that mice are performing differently in individual mazes made us test the hypothesis that differences in cognitive performances in the two land mazes would be accompanied by differences in hippocampal protein levels. C57BL/6J mice were tested in the BM and in the MTM, hippocampi were extirpated 6 h following the probe trials each, and proteins were extracted for gel-based proteomic analysis. Mice learned the task in both paradigms. Levels of hippocampal proteins from several pathways including signaling, chaperone, and metabolic cascades were significantly different between the two spatial memory tasks. Protein levels were linked to spatial memory specifically as yoked controls were used.
多臂迷宫（MTM）和巴恩斯迷宫（BM）是用于评估空间记忆的陆地迷宫。观察到小鼠在个别迷宫中的表现不同，这促使我们验证了一个假设，即两种陆地迷宫中的认知表现差异会伴随着海马体蛋白水平的差异。C57BL/6J 小鼠在 BM 和 MTM 中进行测试，在每个探针试验后 6 小时取出海马体，并提取蛋白质进行凝胶基蛋白质组学分析。小鼠在两种范式中都学会了任务。来自包括信号转导、伴侣和代谢级联在内的多个途径的海马体蛋白水平在两种空间记忆任务之间存在显著差异。由于使用了配对对照，蛋白质水平与空间记忆特异性相关。
