Mishunina T M, Kononenko V Ia
Ukr Biokhim Zh (1978). 1990 Nov-Dec;62(6):71-9.
It has been established that hydrocortisone administration increased the amount of total, free, bound and synaptosomal GABA in the hypothalamus, glutamate decarboxylase activity in the homogenate and synaptosomes and time of the mediator turnover. ACTH administration increased the GABA content and glutamate decarboxylase activity in synaptosomes. The total amino acid content and time of its turnover got higher only with single hormone administration. In the hippocamp hydrocortisone administration increased the total and free GABA contents, its turnover time, glutamate decarboxylase activity in the homogenate and decreased GABA-aminotransferase activity in the homogenate and synaptosomes. The GABA level in synaptosomes grew only with multiple hormone administration. Single administration of ACTH decreased the total GABA content, glutamate decarboxylase activity in the homogenate, while its multiple administration increased the GABA level in synaptosomes followed by a decrease of GABA-aminotransferase activity in the homogenate and synaptosomes. The GABA turnover time fell with single hormone administration and grew with the multiple one. Adrenalectomy induced no changes in the GABA content and activity of its metabolism enzymes in the hypothalamus, however the bound GABA level decreased, while the turnover time increased. In the hippocamp adrenalectomy decreased total, free and synaptosomal GABA contents, glutamate decarboxylase activity in a homogenate and turnover time. Subsequent hydrocortisone administration only partly normalized the revealed changes of the GABA metabolism in the brain structures under adrenalectomy.
已经确定，给予氢化可的松会增加下丘脑总GABA、游离GABA、结合GABA和突触体GABA的含量，增加匀浆和突触体中谷氨酸脱羧酶的活性以及神经递质周转时间。给予促肾上腺皮质激素会增加突触体中GABA含量和谷氨酸脱羧酶活性。仅单次给予激素时，总氨基酸含量及其周转时间会升高。在海马体中，给予氢化可的松会增加总GABA和游离GABA含量、其周转时间、匀浆中谷氨酸脱羧酶活性，并降低匀浆和突触体中GABA转氨酶活性。仅多次给予激素时，突触体中的GABA水平才会升高。单次给予促肾上腺皮质激素会降低总GABA含量、匀浆中谷氨酸脱羧酶活性，而多次给予则会增加突触体中GABA水平，随后降低匀浆和突触体中GABA转氨酶活性。单次给予激素时GABA周转时间缩短，多次给予时则延长。肾上腺切除术未引起下丘脑GABA含量及其代谢酶活性的变化，但结合GABA水平降低，而周转时间增加。在海马体中，肾上腺切除术会降低总GABA、游离GABA和突触体GABA含量、匀浆中谷氨酸脱羧酶活性以及周转时间。随后给予氢化可的松只能部分使肾上腺切除术后大脑结构中GABA代谢的上述变化恢复正常。
