豌豆真核起始因子4E的结晶及X射线初步分析
Crystallization and preliminary X-ray analysis of eukaryotic initiation factor 4E from Pisum sativum.
作者信息
Ashby Jamie A, Stevenson Clare E M, Maule Andrew J, Lawson David M
机构信息
Department of Disease and Stress Biology, John Innes Centre, Norwich NR4 7UH, England.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Aug 1;65(Pt 8):836-8. doi: 10.1107/S1744309109027389. Epub 2009 Jul 30.
Abstract
Crystals of an N-terminally truncated 20 kDa fragment of Pisum sativum eIF4E (DeltaN-eIF4E) were grown by vapour diffusion. X-ray data were recorded to a resolution of 2.2 A from a single crystal in-house. Indexing was consistent with primitive monoclinic symmetry and solvent-content estimations suggested that between four and nine copies of the eIF4E fragment were possible per crystallographic asymmetric unit. eIF4E is an essential component of the eukaryotic translation machinery and recent studies have shown that point mutations of plant eIF4Es can confer resistance to potyvirus infection.
摘要
通过气相扩散法培养了豌豆eIF4E（DeltaN-eIF4E）N端截短的20 kDa片段的晶体。在内部从单个晶体记录了分辨率为2.2 Å的X射线数据。指标与原始单斜对称一致，溶剂含量估计表明每个晶体不对称单元可能有4至9个eIF4E片段拷贝。eIF4E是真核生物翻译机制的重要组成部分，最近的研究表明植物eIF4E的点突变可赋予对马铃薯Y病毒感染的抗性。
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