Sarkissova Y G, Mardanian S S, Haroutunian A V
Institute of Biochemistry, Academy of Sciences of Armenian SSR, Yerevan, USSR.
Biochem Int. 1990 Dec;22(6):977-82.
Chemical modification of tryptophanyl residues of NADPH - adrenodoxin reductase by N - bromosuccinimide and trichloroethanol prevents the interaction of the enzyme with adrenodoxin. The modification does not touch other amino acid residues besides tryptophan (tyrosine, lysine and cysteine) or disturb the structure of protein. The presence of adrenodoxin suppresses the modification. The data obtained indicate the participation of adrenodoxin reductase tryptophan residues in the interaction with adrenodoxin.
N-溴代琥珀酰亚胺和三氯乙醇对NADPH-肾上腺皮质铁氧化还原蛋白还原酶色氨酸残基的化学修饰会阻止该酶与肾上腺皮质铁氧化还原蛋白的相互作用。这种修饰除了色氨酸外不会影响其他氨基酸残基（酪氨酸、赖氨酸和半胱氨酸），也不会扰乱蛋白质的结构。肾上腺皮质铁氧化还原蛋白的存在会抑制这种修饰。所获得的数据表明肾上腺皮质铁氧化还原蛋白还原酶的色氨酸残基参与了与肾上腺皮质铁氧化还原蛋白的相互作用。
