Johnson F B, Leavitt R W, Richards D F
J Clin Microbiol. 1985 Mar;21(3):438-41. doi: 10.1128/jcm.21.3.438-441.1985.
In a comparative study, clinical specimens were cultured for herpes simplex virus (HSV). The presence of virus was noted by the appearance of characteristic cytopathic effect, as determined by standard direct immunofluorescence techniques, by using a direct immunoperoxidase stain for viral antigen, or by using the Selecticult-HSV (SC-HSV) stain for viral antigen. There was 100% correlation between the SC-HSV stain and immunofluorescence staining in recognizing HSV-infected cells (81 of 81 positive specimens). In comparison with observation of cytopathic effect, the SC-HSV system and conventional culture detected 93 and 78% of positive cultures at 48 h postinoculation and 76 and 32%, respectively, at 24 h. By 5 days postinoculation, SC-HSV detected 100% of the positive specimens. As compared with the direct immunoperoxidase stain, SC-HSV stain was slightly more sensitive and gave less background stain. HSV serotypes 1 and 2 were both detected by the SC-HSV stain. The Scott SC-HSV kit appears to be an effective system for the diagnosis of HSV infections.
在一项对比研究中，对临床标本进行单纯疱疹病毒（HSV）培养。通过标准直接免疫荧光技术、使用针对病毒抗原的直接免疫过氧化物酶染色或使用针对病毒抗原的Selecticult-HSV（SC-HSV）染色，根据特征性细胞病变效应的出现来确定病毒的存在。在识别HSV感染细胞方面，SC-HSV染色与免疫荧光染色之间存在100%的相关性（81个阳性标本中的81个）。与观察细胞病变效应相比，SC-HSV系统和传统培养在接种后48小时分别检测到93%和78%的阳性培养物，在24小时分别检测到76%和32%。到接种后5天，SC-HSV检测到100%的阳性标本。与直接免疫过氧化物酶染色相比，SC-HSV染色稍敏感一些，背景染色较少。SC-HSV染色可检测到HSV 1型和2型。Scott SC-HSV试剂盒似乎是诊断HSV感染的有效系统。
