Estación Experimental La Mayora, CSIC, Algarrobo-Costa, Málaga, Spain.
Plant Cell Rep. 2009 Sep;28(9):1411-20. doi: 10.1007/s00299-009-0740-0. Epub 2009 Aug 5.
The effects of indole-butyric acid (IBA) and electro-pulses on rooting and shoot growth were studied in vitro, using olive shoot cultures. Tested shoots were obtained from seedlings belonging to three Spanish cultivars, 'Arbequina', 'Manzanilla de Sevilla' and 'Gordal Sevillana', which have easy-, medium- and difficult-to-root rooting abilities, respectively. The standard two-step rooting method (SRM), consisting of root induction in olive rooting medium supplemented with 0, 0.1 or 1 mg/l IBA followed by root elongation in the same rooting medium without IBA, was compared with a novel one-step method consisting of shoot electro-pulses of 250, 1,250 or 2,500 V in a solution of IBA (0, 0.1 or 1 mg/l) and direct transferral to root elongation medium. The rooting percentage of the seedling-derived shoots obtained with the SRM was 76% for 'Arbequina' and 'Gordal Sevillana' cultivars and 100% for 'Manzanilla de Sevilla' cultivar, whereas with the electro-pulse method, the rooting percentages were 68, 64 and 88%, respectively. IBA dipping without pulse produced 0% rooting in 'Arbequina' seedling-derived shoots. The electroporation in IBA not only had an effect on shoot rooting but also on shoot growth and development, with longer shoots and higher axillary shoot sprouting and growth after some of the treatments. These effects were cultivar-dependent. The electro-pulse per se could explain some of these effects on shoot development.
本研究以橄榄嫩枝离体培养为材料，探讨了吲哚丁酸（IBA）和电脉冲处理对生根和枝梢生长的影响。试验用嫩枝采自 3 个西班牙橄榄品种，即 Arbequina、Manzanilla de Sevilla 和 Gordal Sevillana 的实生苗，这 3 个品种的生根能力分别为易生根、中生根和难生根。标准两步生根法（SRM），即先用含有 0、0.1 或 1mg/L IBA 的橄榄生根培养基诱导生根，然后在不含 IBA 的相同生根培养基中促进生根，与一种新的一步生根法进行了比较。一步法是在含有 0、0.1 或 1mg/L IBA 的溶液中对嫩枝进行 250、1250 或 2500V 的电脉冲处理，然后直接转入生根伸长培养基。SRM 获得的实生苗嫩枝生根率分别为 Arbequina 和 Gordal Sevillana 品种的 76%和 100%，而电脉冲处理的生根率分别为 68%、64%和 88%。IBA 浸泡而不进行电脉冲处理，Arbequina 实生苗嫩枝的生根率为 0%。电穿孔处理不仅影响嫩枝生根，还影响嫩枝的生长发育，一些处理后嫩枝较长，侧枝萌发和生长较多。这些效应具有品种依赖性。电脉冲本身可以解释对嫩枝发育的一些影响。
