10 种西班牙主要橄榄品种木材提取物的酚类成分和抗氧化活性。

Phenolic Components and Antioxidant Activity of Wood Extracts from 10 Main Spanish Olive Cultivars.

机构信息

†Departamento de Quı́mica Inorgánica y Orgánica, Facultad de Ciencias Experimentales, Universidad de Jaén, Campus de Excelencia Internacional Agroalimentario, ceiA3, 23071 Jaén, Spain.

§Biology Department, Baylor University, Box 97388, Waco, Texas 76798, United States.

出版信息

J Agric Food Chem. 2015 Jul 29;63(29):6493-500. doi: 10.1021/acs.jafc.5b02979. Epub 2015 Jul 17.

DOI:10.1021/acs.jafc.5b02979

PMID:26154988

Abstract

The chemical composition and radical-scavenging activity of wood samples from 10 main Spanish olive cultivars were studied. The wood samples were collected during the pruning works from trees growing under the same agronomical and environmental conditions. The 10 ethyl acetate extracts were submitted to HPLC-DAD/ESI-MS analysis to determine the phenolic constituents. Seventeen compounds were identified (10 secoiridoids, 3 lignans, 2 phenol alcohols, 1 iridoid, and 1 flavonoid) by comparison with authentic samples. Significant quantitative and qualitative differences were found among olive cultivars. The lignan (+)-1-hydroxypinoresinol 1-O-β-d-glucopyranoside was the major compound in all olive cultivars, except in cultivars 'Farga' and 'Picual'. The multivariate analysis of all data revealed three sets of cultivars with similar compositions. Cultivars 'Gordal sevillana' and 'Picual' had the most distinct chemical profiles. With regard to the radical-scavenging activity, cultivar 'Picual', with oleuropein as the major phenolic, showed the highest activity (91.4 versus 18.6-32.7%).

摘要

研究了 10 种西班牙主要橄榄品种木材样本的化学成分和自由基清除活性。这些木材样本是在修剪工作中从生长在相同农艺和环境条件下的树木中采集的。将 10 个乙酸乙酯提取物进行 HPLC-DAD/ESI-MS 分析，以确定酚类成分。通过与标准样品进行比较，鉴定出 17 种化合物（10 种裂环烯醚萜、3 种木脂素、2 种酚醇、1 种环烯醚萜和 1 种黄酮类化合物）。橄榄品种之间存在显著的定量和定性差异。除了‘Farga’和‘Picual’品种外，所有橄榄品种中的主要化合物均为木质素(+)-1-羟基松柏醇 1-O-β-d-吡喃葡萄糖苷。对所有数据的多元分析显示，有三组品种具有相似的组成。‘Gordal sevillana’和‘Picual’品种的化学特征最为独特。就自由基清除活性而言，以橄榄苦苷为主要酚类化合物的‘Picual’品种表现出最高的活性（91.4 与 18.6-32.7%）。