Lin Pei-Pei, Hsieh You-Miin, Tsai Cheng-Chih
Department of Food and Nutrition, Providence University, Shalu, Taichung County, Taiwan, ROC.
Anaerobe. 2009 Aug;15(4):122-6. doi: 10.1016/j.anaerobe.2009.01.009.
Enteroaggregative Escherichia coli (EAggEC) infection is an important cause of acute diarrhea, affecting children in developing countries and travelers visiting tropical or subtropical areas. Three probiotics can exert bacteriostatic or bactericidal effects on human and animal intestinal pathogens, the efficiency of probiotics on EAggEC infection remains unclear. In this study, the antagonistic activity of probiotic bacteria isolated from infant faeces was examined against several EAggEC stains. While three isolates, Lactobacillus acidophilus RY2, Lactobacillus salivarius MM1 and Lactobacillus paracasei En4 were shown to significantly inhibit the growth of EAggEC. In addition, the antagonistic activity of the Lactobacillus species was maintained despite heating (100 degrees C, 15 min) of cell free culture supernatant (CFCS). The antagonistic activity of the CFCS however, could be reduced following lactate dehydrogenase treatment and at pH 7.2. Furthermore, in an adhesion-inhibition assay, L. acidophilus RY2 was shown to be more effective than L. salivarius MM1 and L. paracasei EN4. This study suggests that L. acidophilus RY2 could be used as a probiotic organism against EAggEC.
肠聚集性大肠杆菌（EAggEC）感染是急性腹泻的一个重要原因，影响发展中国家的儿童以及前往热带或亚热带地区的旅行者。三种益生菌可对人和动物肠道病原体产生抑菌或杀菌作用，但益生菌对EAggEC感染的效果仍不清楚。在本研究中，检测了从婴儿粪便中分离出的益生菌对几种EAggEC菌株的拮抗活性。结果显示，嗜酸乳杆菌RY2、唾液乳杆菌MM1和副干酪乳杆菌En4这三株分离菌可显著抑制EAggEC的生长。此外，尽管对无细胞培养上清液（CFCS）进行加热（100℃，15分钟）处理，乳杆菌属的拮抗活性仍得以维持。然而，CFCS的拮抗活性在乳酸脱氢酶处理后以及pH值为7.2时会降低。此外，在黏附抑制试验中，嗜酸乳杆菌RY2显示出比唾液乳杆菌MM1和副干酪乳杆菌EN4更有效。本研究表明，嗜酸乳杆菌RY2可作为针对EAggEC的益生菌。
