Rideout B A, Moore P F, Pedersen N C
Department of Medicine, School of Veterinary Medicine, University of California, Davis.
Tissue Antigens. 1990 Nov;36(5):221-7. doi: 10.1111/j.1399-0039.1990.tb01832.x.
A new monoclonal antibody raised against gradient-purified feline immunodeficiency virus was found to recognize a bimolecular complex, comprising 27-29 kD and 32-35 kD subunits, on feline peripheral blood lymphocytes. Immunoperoxidase staining of feline tissues with this antibody, designated 43.2H2, demonstrated a reactivity pattern similar to that described for MHC II antigens of the dog, horse, and pig, but differed from human and mouse in having staining of T-cell zones in spleen and lymph nodes. Flow cytometric analysis revealed that 42.3H2 reacted with 88.97% +/- 16.00% of feline peripheral blood lymphocytes (n = 20). This high level of reactivity was found to be consistent by repeated sampling over a 4-month period. Two-color flow cytometric analysis was used to determined the reactivity pattern on lymphocyte subsets: 88.92% +/- 7.30% of CD4+ lymphocytes were 42.3H2-positive, while 85.99% +/- 11.46% of CD8+ cells were positive (n = 11 for both). B lymphocytes had the highest reactivity (99.47% +/- 0.45; n = 9) and also had the highest fluorescence intensity. By gating based on light scatter properties, 95.06% +/- 7.35% of monocytes were 42.3H2-reactive (n = 18), while granulocytes were negative.
发现一种针对梯度纯化的猫免疫缺陷病毒产生的新型单克隆抗体，可识别猫外周血淋巴细胞上一种由27 - 29 kD和32 - 35 kD亚基组成的双分子复合物。用这种名为43.2H2的抗体对猫组织进行免疫过氧化物酶染色，显示出与狗、马和猪的MHC II抗原所描述的反应模式相似，但与人和小鼠不同，在脾脏和淋巴结的T细胞区有染色。流式细胞术分析显示，42.3H2与88.97% ± 16.00%的猫外周血淋巴细胞发生反应（n = 20）。发现在4个月的时间内重复采样，这种高反应性是一致的。采用双色流式细胞术确定淋巴细胞亚群上的反应模式：88.92% ± 7.30%的CD4 + 淋巴细胞为42.3H2阳性，而85.99% ± 11.46%的CD8 + 细胞为阳性（两者n = 11）。B淋巴细胞具有最高的反应性（99.47% ± 0.45；n = 9），并且荧光强度也最高。通过基于光散射特性进行门控，95.06% ± 7.35%的单核细胞对42.3H2有反应（n = 18），而粒细胞为阴性。
