Hunt P, McConnell I, Grant C K, Else R W, Hopkins J
Department of Veterinary Pathology, University of Edinburgh, Royal (Dick) School of Veterinary Studies, Summerhall.
Res Vet Sci. 1995 Nov;59(3):195-200. doi: 10.1016/0034-5288(95)90001-2.
This paper describes the characterisation of six independently produced monoclonal antibodies (mAbs) specific for non-polymorphic determinants of feline major histocompatibility complex (MHC) class II. One mAb is an anti-sheep class II which cross-reacts with the cat and five have been produced in response to immunisation with purified feline immunodeficiency virus. Despite their independent source all the mAbs have identical reactivities, immunoprecipitating two complex groups of polypeptides of M(r) 33 to 36.000 (MHC class II alpha chains) and M(r) 28 to 31,000 (MHC class II beta chains). Immunoblot analysis showed them to be beta chain-specific. One and two-dimensional electrophoresis revealed the complexity of feline class II mass and charge and implied the expression of multiple class II loci in the cat. Furthermore, it was demonstrated that distinct cell populations expressed a distinct range of class II variants. This suggesting either the differential expression or the distinct post-translational modification of lymphocytes from different sites. The mAbs have also been used for the detailed examination of the cellular distribution and tissue localisation of MHC class II in the cat.
本文描述了六种针对猫主要组织相容性复合体（MHC）II类非多态性决定簇独立产生的单克隆抗体（mAb）的特性。一种mAb是抗绵羊II类抗体，可与猫发生交叉反应，另外五种是在用纯化的猫免疫缺陷病毒免疫后产生的。尽管它们来源独立，但所有mAb都具有相同的反应性，能免疫沉淀两组分子量分别为33至36,000（MHC II类α链）和28至31,000（MHC II类β链）的复杂多肽。免疫印迹分析表明它们是β链特异性的。一维和二维电泳揭示了猫II类分子在质量和电荷方面的复杂性，并暗示猫中存在多个II类基因座的表达。此外，还证明了不同的细胞群体表达不同范围的II类变体。这表明来自不同部位的淋巴细胞存在差异表达或不同的翻译后修饰。这些mAb还被用于详细研究猫中MHC II类分子的细胞分布和组织定位。
