Ghandilyan Artak, Barboza Luis, Tisné Sébastien, Granier Christine, Reymond Matthieu, Koornneef Maarten, Schat Henk, Aarts Mark G M
Laboratory of Genetics, Wageningen University, Arboretumlaan 4, NL-6703 BD Wageningen, the Netherlands.
Current address: Centro para Investigaciones en Granos y Semillas (CIGRAS), Universidad de Costa Rica, San José, Costa Rica.
New Phytol. 2009;184(1):180-192. doi: 10.1111/j.1469-8137.2009.02953.x. Epub 2009 Jul 27.
Rosettes of 25 Arabidopsis thaliana accessions and an Antwerp-1 (An-1) x Landsberg erecta (Ler) population of recombinant inbred lines (RILs) grown in optimal watering conditions (OWC) and water deficit conditions (WDC) were analysed for mineral concentrations to identify genetic loci involved in adaptation of mineral homeostasis to drought stress. Correlations between mineral concentrations were determined for accessions and a quantitative trait locus (QTL) analysis was performed for the RIL population. Plant growth and rosette mineral contents strongly decreased in WDC compared with OWC. Mineral concentrations also generally decreased, except for phosphorus (P), which remained constant, and potassium (K), which increased. Large variations in mineral concentrations were observed among accessions, mostly correlated with total rosette leaf area. Mineral concentration QTLs were identified in the RIL population, but only a few were common for both conditions. Clusters of mineral concentration QTLs often cosegregated with dry weight QTLs. Water deficit has a strong effect on rosette mineral status. This is genetically determined and seems largely a pleiotropic effect of the reduction in growth. The low number of common mineral concentration QTLs, shared among different RIL populations, tissues and conditions in Arabidopsis, suggests that breeding for robust, mineral biofortified crops will be complex.
对25个拟南芥生态型以及安特卫普-1（An-1）×直立型兰茨贝格（Ler）重组自交系（RIL）群体在最佳浇水条件（OWC）和水分亏缺条件（WDC）下生长的莲座叶进行了矿物质浓度分析，以确定参与矿物质稳态适应干旱胁迫的基因位点。测定了生态型之间矿物质浓度的相关性，并对RIL群体进行了数量性状基因座（QTL）分析。与OWC相比，WDC下植物生长和莲座叶矿物质含量显著下降。除了保持恒定的磷（P）和增加的钾（K）外，矿物质浓度总体上也有所下降。在生态型之间观察到矿物质浓度存在很大差异，这大多与莲座叶总面积相关。在RIL群体中鉴定出了矿物质浓度QTL，但两种条件下只有少数是共同的。矿物质浓度QTL簇通常与干重QTL共分离。水分亏缺对莲座叶矿物质状态有强烈影响。这是由基因决定的，似乎在很大程度上是生长减少的多效性效应。拟南芥不同RIL群体、组织和条件之间共享的共同矿物质浓度QTL数量较少，这表明培育健壮的、矿物质生物强化作物将很复杂。
