传统猪群中腹泻猪的轮状病毒中典型毒株和非典型毒株的相对流行率。

Relative prevalence of typical and atypical strains among rotaviruses from diarrheic pigs in conventional swine herds.

作者信息

Janke B H, Nelson J K, Benfield D A, Nelson E A

机构信息

South Dakota Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings 57007.

出版信息

J Vet Diagn Invest. 1990 Oct;2(4):308-11. doi: 10.1177/104063879000200410.

DOI:10.1177/104063879000200410

PMID:1965637

Abstract

Polyacrylamide gel electrophoresis was conducted on genomic RNA extracted from rotaviruses detected in diarrheic pigs from conventional swine herds. Ninety samples contained sufficient virus for RNA band visualization and genome classification. Genome profiles were characteristic of typical group A rotaviruses in 67.8% of the 90 samples, of group B rotaviruses in 10.0%, and of group C rotaviruses in 11.1%. In 11.1% of the samples, the presence of more than 11 bands suggested concurrent infection with more than 1 strain of rotavirus. In infections among nursing pigs, 76.4% were group A rotaviruses, 7.4% were group B, 7.4% were group C, and 8.8% were coinfections. In infections among weaned pigs, 40.9% were group A, 18.2% were group B, 22.7% were group C, and 18.2% were coinfections. Coelectrophoresis with prototype OSU and Gottfried strains revealed a great diversity in electropherotype among field strains of rotavirus.

摘要

对从传统猪群腹泻猪中检测到的轮状病毒提取的基因组RNA进行聚丙烯酰胺凝胶电泳。90个样本含有足够的病毒用于RNA条带可视化和基因组分类。在90个样本中，67.8%的基因组图谱具有典型A组轮状病毒特征，10.0%具有B组轮状病毒特征，11.1%具有C组轮状病毒特征。在11.1%的样本中，超过11条带的出现表明同时感染了1种以上的轮状病毒株。在哺乳仔猪感染中，76.4%为A组轮状病毒，7.4%为B组，7.4%为C组，8.8%为混合感染。在断奶仔猪感染中，40.9%为A组，18.2%为B组，22.7%为C组，18.2%为混合感染。与原型OSU和Gottfried毒株进行共电泳显示，轮状病毒野毒株的电泳型存在很大差异。