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具有非典型电泳图谱的A群猪轮状病毒的分离

Isolation of group A swine rotaviruses displaying atypical electropherotypes.

作者信息

Bellinzoni R C, Mattion N M, Burrone O, Gonzalez A, La Torre J L, Scodeller E A

出版信息

J Clin Microbiol. 1987 May;25(5):952-4. doi: 10.1128/jcm.25.5.952-954.1987.

DOI:10.1128/jcm.25.5.952-954.1987
PMID:3034969
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC266131/
Abstract

Swine rotaviruses displaying distinctive electropherotypes were isolated from the feces of diarrheic piglets in two swine herds in the province of Buenos Aires, Argentina. In one case all samples isolated showed abnormal electropherotypes. All samples were classified as group A reactive when assayed by an enzyme-linked immunosorbent assay. Three samples from this herd were adapted to grow in tissue culture. The electrophoretic pattern of the genomic RNA as well as the group A reactivity of one of these viruses was retained after cloning in MA-104 cells. In the other pig unit were found samples displaying both classical and abnormal electropherotypes. These viruses were also positive in the enzyme-linked immunosorbent assay; however, since they could not be adapted to grow in tissue culture, this classification must be considered tentative. The abnormal electropherotype exhibited by these pig viruses strongly resembles those of human origin called super short.

摘要

从阿根廷布宜诺斯艾利斯省两个猪群腹泻仔猪粪便中分离出显示独特电泳图谱型的猪轮状病毒。在一个案例中,所有分离出的样本均显示异常电泳图谱型。通过酶联免疫吸附测定法检测时,所有样本均被归类为A组反应性。从该猪群中选取的三个样本经适应后可在组织培养中生长。其中一种病毒在MA - 104细胞中克隆后,其基因组RNA的电泳图谱以及A组反应性得以保留。在另一个猪单位中发现了显示经典和异常电泳图谱型的样本。这些病毒在酶联免疫吸附测定中也呈阳性;然而,由于它们无法适应在组织培养中生长,因此这种分类必须被视为暂定。这些猪病毒所呈现的异常电泳图谱型与人类来源的所谓超短型极为相似。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62ef/266131/e45b1a5f8f1e/jcm00089-0217-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62ef/266131/e45b1a5f8f1e/jcm00089-0217-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62ef/266131/e45b1a5f8f1e/jcm00089-0217-a.jpg

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